熊果酸对大鼠子宫平滑肌收缩影响.docVIP

熊果酸对大鼠子宫平滑肌收缩影响 　　　　　　　　　作者：马力扬，李志强，徐敬东 【关键词】; 熊果酸；子宫；平滑肌 毕业论文 　　摘要：目的; 观察熊果酸对离体大鼠子宫活动的影响。方法; 将离体子宫肌条在1g的前负荷下温育，加入熊果酸1.0times;10-8、2.0times;10-8、3.0times;10-8 、4.0times;10-8g/L，以等容量生理盐水作为自身对照，记录30min；分别使用不同的受体拮抗剂5min后，再加入熊果酸3.0times;10-8g/L。用Biolap 410生物机能仪记录熊果酸对离体子宫平滑肌运动的影响。结果; 熊果酸可增强离体子宫平滑肌的收缩振幅、频率和持续时间，熊果酸剂量与子宫肌兴奋作用存在剂量反应关系。并使用受体阻断剂苯海拉明(2times;10-6mol/L)、酚妥拉明(2times;10-6mol/L)、异博定(2times;10-7mol/L)及消炎痛(2times;10-5mol/L)观察其改变。该增强作用可被消炎痛阻断，而异博定、酚妥拉明、苯海拉明未能阻断熊果酸的作用。结论; 熊果酸增强作用与前列腺素酶的合成与释放有关，不通过Ｌ型电压依赖性钙通道、alpha;受体以及H1受体发挥作用。 毕业论文 　　关键词：熊果酸；子宫；平滑肌 毕业论文 　　Effect of ursolicacid on contractile activity of uterine　smooth muscle strips of virginal rats 毕业论文 　　ABSTRACT: Objecitve; To observe the effects of ursolicacid on contractile activity of uterine smooth muscle strips of virginal rats. Methods; 20 Wistar virginal rats, weight 200-300g were selected, to record the effects of ursolicacid (1.0times;10-8, 2.0times;10-8, 3.0times;10-8, 4.0times;10-8g/L) on contractile activity of uterine smooth muscle strips with Biolap 410 Biological System. Diphenhydramine (2times;10-6mol/L), Phentolamine (2times;10-6mol/L), Verapamil(2times;10-7mol/L), Indomethacin (2times;10-5mol/L) were used as antagonists to study the mechanisms of ursolicacid on uterine smooth muscle strips. Results; Ursolicacid could significantly potentiate uterine contractile activity of virginal rats. It could increase the frequencies, prolong the duration and enlarge the amplitude of contractile activity. The effect of which could be inhibited by the Indomethacin; but could not restrained by other antagonists. Conclusion; The results of Ursolicacid may be mainly primarily with prostaglandin synthese or release, but not mediated by H1 receptor, alpha; receptor and Lvoltagedependant calcium channels. 毕业论文 　　KEY WORDS: ursolicacid; uterine; smooth muscle 毕业论文 　　熊果酸(ursolicacid)又名乌索酸、乌苏酸，属三萜类化合物，相对分子质量为456，在自然界分布很广，存在于杜鹃花科植物熊果的叶、果实，玄参植物毛泡桐的叶，茜草科植物栀子的果实，龙胆科植物湿生蕾，木樨科植物女贞的叶，紫威科植物毛子草的地上部分。熊果酸具有广泛的生物学效应，其突出