牛膝提取物神经再生素促小鼠坐骨神经再生实验探究.doc

牛膝提取物神经再生素促小鼠坐骨神经再生实验探究 　【摘要】 　　目的观察中药牛膝提取物神经再生素(NRF)对小鼠损伤坐骨神经的修复作用。方法ICR小鼠50只，雌雄各半，行坐骨神经夹伤术，随机分成5组：NRF高、中、低剂量组，弥可保组（阳性对照），生理盐水组（空白对照）。术后每日腹腔注射给药。采用小鼠坐骨神经功能指数(sciatic function index, SFI)、组织形态学及电镜检查，观察NRF对损伤坐骨神经的影响。结果与空白对照组相比，NRF可显著改善小鼠的坐骨神经功能。超微结构观察表明，实验组有髓神经纤维的髓鞘形态、厚度、成熟度均优于对照组，而变性纤维的数目少于对照组。结论NRF能促进小鼠坐骨神经损伤后的修复及其功能的恢复。 【关键词】 牛膝； 神经再生素； 坐骨神经损伤； 神经再生 　　Abstract：ObjectiveTo investigate the repair effect of nerve regeneration factor（NRF）extracted from Achyranthes bidentata Blume (BL) on the crushed sciatic nerve of mouse. MethodsFifty ICR mice suffered from the sciatic nerves crush were used in the experiment. The animals were randomly divided into five groups: three NRF groups (low, middle and high dose), Mecobalamin group (positive control) and blank control group. These mice after sciatic nerves crush received NRF by intraperitoneal injection every day. Sciatic function index (SFI) was tested every 4 days. Histomorphology and ultra-structure were used to the study on the 21th day. ResultsCompared with the black control group, SFI in NRF group and the section area of the gastrocnemius muscle were larger. The number of regeneration nerve fibers, thick and degree of maturity of regeneration myelinated nerve sheath in NRF groups were more than those in the blank control group. ConclusionNRF can promote the regeneration of peripheral nerve and improve the recovery of function. 　　Key words：Achyranthes bidentata Blume (BL)； Nerve regeneration factor； Sciatic nerve injury； Nerve regeneration 　　神经再生素（nerve regeneration factor, NRF）是从中药怀牛膝中提取的有效组分（中国专利申请号:200710019780.4），主要成分为甾酮、多糖、多肽等。已有的研究资料显示，神经再生素具有促进神经元生长、防止神经元凋亡的作用［1～6］。本实验采用小鼠坐骨神经损伤模型，观察NRF对周围神经损伤的修复作用，进一步探讨其药理作用。 　　1 材料与方法 　　1.1 动物和试剂 健康成年ICR小鼠50只(由南通大学动物中心提供)，体重（20±2）g，雌雄各半，随机分成5组。NRF由南通大学神经再生重点实验室提供（批号：060213），注射用生理盐水溶解至适当浓度备用。弥可保注射液：日本卫材株式会社制造（批号：050665）。 　　1.2 模型制备 复合麻醉剂（0.03 ml/10 g）作腹腔麻醉，左臀部作1 cm切口，在距梨状肌下缘0.3 cm处以特制钳子，钳夹坐骨神经3次，10 s/次，间隙10 s，挤压损伤的宽度为2 mm。损伤远端以8-0显微缝线在神经外膜上作标记，缝合切口。术后每天腹腔注射给药：NRF高、中、低剂量组（0.1