环肽a-amanitin对人肝癌细胞SMMC-7721体外毒性作用探究.docVIP

环肽a-amanitin对人肝癌细胞SMMC-7721体外毒性作用探究 　 作者：曹玫，颜亮，聂远洋，冯甦，张杰，侯若彤，孙群，杨志荣，赵建 【摘要】 目的研究毒蕈环肽a-amanitin对人肝癌细胞SMMC-7721的体外毒性作用。方法运用MTT法研究α-amanitin不同浓度和不同作用时间对体外传代培养的人肝癌细胞SMMC-7721的抑制增殖作用，再进行环肽α-amanitin对细胞毒性作用的数学模型拟合。 结果研究发现，α-amanitin的使用剂量与细胞存活率存在负相关。7.12×10-4 mmol/L 和7.12×10-10 mmol/L α-amanitin对SMMC-7721作用24 h后，细胞存活率分别为71.25%和87.47%，作用效果分别与10 mmol/L和2.5 mmol/L 环磷酰胺相似。SMMC-7721细胞存活率平方根的反正弦值与α-amanitin浓度的对数变换值的相关和回归分析结果显示二者显著性相关，曲线拟合结果显示三次曲线模型拟合最好，回归方程为Y=31.257 7-12.902X-1.5117X2-0.0616X3。对SMMC-7721细胞存活率平方根的反正弦值与α-amanitin作用时间进行相关和回归分析，二者显著性相关，三次曲线模型拟合最好，对应回归方程为Y=89.668 5-8.616 6X+1.791X2-0.1271X3。结论环肽a-amanitin对人肝癌细胞SMMC-7721体外增殖有显著抑制作用，且单位摩尔数的α-amanitin抑制作用远强于单位摩尔数的环磷酰胺。 【关键词】 α-amanitin; SMMC-7721; 细胞毒性 　　Abstract：ObjectiveTo study the inhibitory effect of cyclopeptide α-amanitin on human hepatoma SMMC-7721 cells. MethodsThe SMMC-7721 cells were treated with different concentration and duration of α-amanitin. The cell survival rate (CRS) of SMMC-7721 cells was examined by MTT assay in vitro, and the relative results were fitted with mathematical model. ResultsThe cell survival rate was reversely correlated with the concentration of α-amanitin. The cell growth was restrained strikingly when the cells were treated by 7.12×10-10 mmol/L α-amanitin for 24 h. The cell survival rate were 71.25% and 87.47% after treating with 7.12×10-4 mmol/L and 7.12×10～10 mmol/L α-amanitin for 24 h, respectively. The arcsine transformation of the CSR square root and the logarithmic transformation of α-amanitin concentration were correlated and regressed. The curve fitting showed that the cubic model was the best one. The regression equation was Y=31.257 7-12.902X-1.511 7X2-0.061 6X3. The arcsine transformation of the CSR square root and the action time of α-amanitin were correlated and regressed. The correlation was also significant and the cubic model was the best one. The cubic regression equation was Y=89.668 5-8.6166X+1.791X2-0.1271X3. ConclusionThe cyclopeptide α-amanitin has significant