电针丰隆穴对高脂血症大鼠一氧化氮及内皮素影响.docVIP

电针丰隆穴对高脂血症大鼠一氧化氮及内皮素影响 　作者：周利, 张红星, 刘灵光, 万文俊 【摘要】 探讨电针（electro-acupuncture, EA）丰隆穴对高脂血症（hyperlipidemia, HLP）大鼠血脂、一氧化氮（nitric oxide, NO）及内皮素（endothelin, ET）含量的影响。方法：将80只Wistar大鼠随机分为4组：正常对照组（饲喂基础饲料）、模型组（饲喂高脂饲料）、电针治疗组（饲喂高脂饲料+电针丰隆治疗）、普伐他汀治疗组（饲喂高脂饲料+普伐他汀钠灌胃）。各组20只。30 d后称取各组大鼠体质量，以改良大鼠心脏穿刺采血方法采血，检测血脂、NO以及ET含量。 结果：与正常对照组比较，模型组大鼠体质量、总胆固醇（total cholesterol, TC）、甘油三酯（triacylglycerol, TG）、低密度脂蛋白胆固醇（low density lipoprotein-cholesterol, LDL-C）和ET水平显著升高（P＜0.05，P＜0.01）；高密度脂蛋白胆固醇（high density lipoprotein-cholesterol, HDL-C）、NO水平显著降低（P＜0.05）。与模型组比较，普伐他汀治疗组、电针治疗组大鼠体质量、TC、TG、LDL-C水平明显降低（P＜0.01），NO水平显著升高。普伐他汀治疗组HDL-C水平显著升高，而电针治疗组升高不明显，两组之间差异有统计学意义（P＜0.01）。普伐他汀治疗组ET水平降低（P＜0.05），电针治疗组ET水平降低不明显（P＞0.05）。结论：电针治疗和西药治疗均对HLP大鼠血脂具有良性调节作用，且能在一定程度上纠正高脂状态下NO和ET水平的失衡。西药对HLP大鼠HDL-C有较好的良性调节作用，而电针对HLP大鼠HDL-C的调节作用有限。 【关键词】 高脂血症; 电针疗法; 丰隆; 一氧化氮; 内皮素 Objective: To investigate the effect of electro-acupuncture (EA) at Fenglong (GV 16) on body weight, blood lipids, nitric oxide (NO) and endothelin (ET) in rats with hyperlipidemia (HLP). Methods: Eighty Wistar rats were randomly divided into normal control group (fed normal diet), untreated group (fed a high-fat diet), EA-treated group (fed a high-fat diet plus EA therapy) and pravastatin-treated group (fed a high-fat diet plus pravastatin tablet). There were 20 rats in each group. The body weight and the blood content of total cholesterol (TC), triacylglycerol (TG), high density lipoprotein-cholesterol (HDL-C), low density lipoprotein-cholesterol (LDL-C), NO the and ET of the rats in different groups were measured before experiment and after 30-day treatment. A modified method of cardiac puncture for blood sampling was used for blood collection. Results: Compared with the normal control group, the body weight and the levels of TC, TG, LDL-C and ET in the untreated group were significantly elevated (P＜0.01, P＜0.05), while the levels of HDL-C and NO were obviously decreased (P＜0.05). The body weight and the levels of TC, TG, LDL-C demonstrated significan