白细胞预激及非适应性反应实验探究.docVIP

白细胞预激及非适应性反应实验探究 　【摘要】 目的 观察中性粒细胞粘附活性和杀伤效应在大鼠严重创伤后的动态变化规律，探讨中性粒细胞致敏与活化不同功能状态下的生物学标记对创伤的预警意义。方法 采用多处骨折和广泛软组织挫伤建立大鼠严重创伤模型，分别于创伤后2、4、8、12、24h不同时相点采集血标本；攻击组于体外LPS攻击PMN模拟“二次打击”；检测各组PMN释放O2-和PMN-EC的粘附活性的变化。结果 创伤组2、4h时相点中性粒细胞体外自发释放O2-自由基量和PMN-EC粘附活性与基础表达值无明显改变，8、12、24h时相点明显增多（Plt;0.05），而8、12、24h时相点则显示上述指标均较对照组明显增加；LPS攻击组显示各相应时相点O2-自由基释放量、PMN-EC粘附百分率均较其对照组呈不同程度升高；尤其是2、4h时相点较同组其他时相点增加更为明显（Plt;0.01），且较创伤组明显增加（Plt;0.01）。结论 创伤早期PMN处于致敏状态，表现为活性氧的释放能力显著增加和PMN-EC的粘附活性明显上调。 【关键词】 创伤 中性粒细胞 预激 致敏 活化 内毒素 大鼠 【Abstract】 Objective To observate the changes of release O2- of PMN and PMN-EC adhesion activity at rats with multiple trauma.It’s valuable that providing theoretical instruction for clinical treatment,and expecting a new road for prevention and treatment of severe injury by exploring the mechanism and biomarker of PMN priming.Methods Adopting murine modles of multiple broken-bone and soft tissue injury, the basal levels of PMN O2- release were found in unstimulated PMN harvested from health rats. Systemic blood was collected at 2、4、8、12、24h after trauma. With LPS challenge the PMN harvested at various times in vitro（second hit）. The methods used in the study are as follows: 1. In vitro release O2- of PMN was messured by ultraviolet spectroscopy. Results PMN-EC adhesion activity and spontaneous in vitro O2- release by PMN harvested from trauma rats at 2 and 4hours after injury was also quite low.In contrast, which modestly increased at 8,12 and 24 hours after injury (Plt;0.05), the above changes all remained higher than that of control group at 8,12 and 24hours after injury. Also, LPS challenge PMNs that expressed more O2-than control,especialy significant elevations at 2 hours、4 hours after injury,compared with control group,PMN-EC adhesion activity all were markedly enhanced at various times after injury,especialy the denotes data at 2 and 4 hours,that are significantly different (Plt;0.01) from the other various times. Conclusion The injury to PMN was a role of priming early t