白英水提物诱导人胃癌SGC-7901细胞凋亡实验探究.doc

白英水提物诱导人胃癌SGC-7901细胞凋亡实验探究 　 作者：吴剑，李华，涂硕，余乐涵，万福生 【摘要】 　　目的观察白英水提物（SLT）对胃癌SGC-7901细胞凋亡及相关基因表达的影响。方法常规法制备白英水提物；实验分正常对照组、白英处理组和顺铂处理组。白英处理组加入白英水提物终浓度分别为12.5，25和50 mg/ml。MTT法检测细胞增殖抑制率，荧光显微镜观察SGC-7901细胞凋亡及形态变化，半定量RT-PCR分析基因bcl-xl，bid，caspase-9 和caspase-3 mRNA表达。结果与正常对照组比较，3个剂量白英处理组SGC-7901细胞增殖抑制均有显著性增加，且呈明显的时效和量效关系，细胞凋亡呈显著性增多（Plt;0.05），表现细胞核固缩、染色质凝集、边聚等；基因bcl-xl mRNA表达显著性下降（Plt;0.05）， bid、caspase-9 和caspase-3 mRNA表达有显著性升高（Plt;0.05），并随白英浓度增加而加强。结论白英能剂量依赖性的抑制SGC-7901细胞增殖和诱导凋亡，其作用可能与调控bcl-xl、bid、caspase-9和caspase-3 mRNA表达相关。 【关键词】 白英； SGC-7901细胞； 凋亡； 基因表达 　 　　Abstract：ObjectiveTo investigate the effects of Solanum lyratum Thunb. Extract (SLT) on apoptosis of human stomach cancer SGC-7901 cells.MethodsDried whole herbs of Solanum Lyratum Thunb were extracted by boiling distilled water. Human stomach cancer SGC-7901 cells were randomly divided into control group, SLT-treated groups (12.5 mg/ml, 25 mg/ml, 50 mg/ml ) and positive control(25μg/ ml DDP ). The growth inhibitory rate was evaluated by MTT assay. Morphological changes of apoptosis were observed with fluorescence microscope. Cell apoptosis rate was determined by flow cytometry and the express of bcl-xl , caspase-9 ,caspase-3 and bid mRNA were detected by semi-quantity RT-PCR.ResultsCompared with control group, the cell proliferation inhibitory rate and apoptosis rate of Human stomach cancer SGC-7901 cells were increased obviously (Plt;0.05) in SLT-treated groups. Morphology of SGC-7901 cells showed the nuclear shrinkage, chromatin condensation and margination, and no changes were observed in control group. The expression of bcl-xl mRNA was obviously decreased (Plt;0.05), the expression of caspase-9 ,caspase-3 and bid mRNA were obviously increased respectively (Plt;0.05), in a dose-dependent manner in SGC-7901 cells of the SLT-treated groups. ConclusionSLT can induce apoptosis and inhibit proliferation of the human stomach cancer SGC-7901 cells,by regulating expression of bcl-xl, caspase-9 ,caspase-3 and bid genes. 　　K