白藜芦醇对肝癌细胞基质金属蛋白酶9表达影响.doc

白藜芦醇对肝癌细胞基质金属蛋白酶9表达影响 　 作者：余海波, 潘承恩, 吴武军, 赵四海, 张慧峰 【摘要】 目的：观察白藜芦醇（resveratrol, Res）对体外人肝癌细胞系SMMC7721细胞增殖及基质金属蛋白酶9（matrix metalloprotEinase9, MMP9）表达的影响，初步探讨Res抑制肝癌细胞侵袭转移的机制。方法：应用不同浓度的Res分别作用于人肝癌细胞系SMMC7721细胞24、48和72 h，采用甲基噻唑基四唑比色试验（methyl thiazolyl tetrazolium, MTT）观察Res对SMMC7721细胞增殖的影响；反转录聚合酶链反应（reverse transcription polymerase chain reaction, RTPCR）检测MMP9 mRNA的表达；蛋白印迹分析（Western blot analysis）法检测MMP9蛋白表达情况。结果：Res对SMMC7721细胞有增殖抑制作用，且呈量效和时效关系；经Res作用后，SMMC7721细胞MMP9 mRNA和蛋白表达均明显下降（P＜0.05）。结论：Res对肝癌细胞的生长具有抑制作用，且能下调其MMP9的表达，可能具有抑制肝癌细胞侵袭转移的作用。 【关键词】 白藜芦醇; 肝癌; 基质金属蛋白酶9 毕业论文 　　Objective: To observe the effects of resveratrol on proliferation of human hepatocellular carcinoma cell line SMMC7721 cells and expression of matrix metalloprotEInase9 (MMP9) in vitro. 　　Methods: SMMC7721 cells were treated with different concentrations of resveratrol for 24, 48 and 72 h, respectively. The effect of resveratrol on proliferation of SMMC7721 cells was assessed with methyl thiazolyl tetrazolium (MTT). The expression of MMP9 mRNA was determined by reverse transcription polymerase chain reaction (RTPCR). MMP9 protein was identified by Western blot analysis. 　　Results: Resveratrol could inhibit the proliferation of SMMC7721 cells with dose and timedependent effects. Moreover, both MMP9 mRNA expression and MMP9 protein production were markedly reduced after resveratrol treatment. 论文代写 　　Conclusion: Resveratrol can inhibit the proliferation of SMMC7721 cells and downregulate MMP9 expression. It is presumed that resveratrol may suppress the invasion and metastasis of hepatocellular carcinoma. 　　Keywords: resveratrol; liver cancer; matrix metalloproteinase9 　　原发性肝癌是最常见的消化道恶性肿瘤之一，术后5年生存率低，仅为25%～39%［1］。侵袭转移是影响肝癌预后的主要因素。因此，阐明肝癌侵袭转移的发生机制，寻求有效的抗侵袭转移 治疗 措施，对改善肝癌患者预后具有重要意义。白藜芦醇（resveratrol, Res）是一种从葡萄、虎杖等植物中分离提取的一种多酚类化合物。近年来的研究表明，Res有抗炎、抗氧化、改善微循环和调节脂质代谢等众多生物学活性。深入研究发现其具有抗肿瘤作用［2］，特别是对于肝癌细胞，Res有明显的抑制增殖和诱导凋亡的作用，但目前鲜有抑制肝癌侵袭转移的报道。本实验通过研究Res影响肝癌细