肾气丸“阴中求阳”配伍对肾阳虚大鼠下丘脑多巴胺及五羟色胺含量.docVIP

肾气丸“阴中求阳”配伍对肾阳虚大鼠下丘脑多巴胺及五羟色胺含量 　 作者：吴勇，李政木，卓廉佳，陈炜璇，范明威 【摘要】 　　目的用肾气丸各拆方组水煎液灌胃肾阳虚大鼠，测定灌胃后各组大鼠下丘脑中多巴胺（DA）、五羟色胺（5-HT）的含量，分析肾气丸“阴中求阳”治疗肾阳虚证的机理。方法以氢化可的松25 mg/(kg·d）注射大鼠，连续12 d，同时以各肾气丸拆方组水煎液灌胃，于灌胃第21天，麻醉大鼠，取出下丘脑，称重，以5%高氯酸匀浆，高速低温离心后分取上清液，用高效液相色谱法检测DA，5-HT的含量。结果与正常组比较，模型组大鼠下丘脑中DA、5-HT含量升高，经灌胃给药后，各拆方组大鼠下丘脑中DA，5-HT的含量均有不同程度的降低，以阴阳双补组含量最低，而补阳组降低不明显，其中阴阳双补1组含量低于补阳1组，阴阳双补2组含量低于补阳2组,具有统计学意义。结论降低下丘脑中多巴胺、五羟色胺的含量，可能是肾气丸“阴中求阳”配伍治疗肾阳虚证的机理之一。 【关键词】 肾气丸 阴中求阳 肾阳虚证 多巴胺 五羟色胺 高效液相色谱法 　　Abstract：ObjectiveTo study the influence of compatibility of Shenqi Pill “Reinforcing Yang from Yin” on the contents of DA,5-HT in hypothalamus of deficient kidney-yang Rats.MethodsThe rats were injected with cortisone at the dose of 25 mg/(kg·d) for consecutive 12 days and adminstered with dismantled decoction at the same time. On the 21st day, the rats were sacrificed and the contents of DA ,5-HT in hypothalamus were measured by the method of high performance liquid chromatogram. ResultsCompared with normal group, the contents of DA,5-HT was increased in model group. After intragastric administration, the content of DA,5-HT in each dismantled group were decreased at different level in hypothalamus. The first group of invigorating yin-yang was higher than the first group of invigorating yang, the second group of invigorating yin-yang was higher than the second group of invigorating yang, and all of these had statistic significance. ConclusionThe decrease of DA,5-HT contents in hypothalamus is likely to be the mechanism by which Shenqi Pill treats the kidney-yang asthenia syndrome by the compatibility of “reinforcing yang from yin”. 　　Key words：Shenqi Pill; Reinforcing yang from yin; Kidney-yang asthenia syndrome; DA； 5-HT; HPLC 　　肾气丸是临床治疗肾阳虚证的名方，方由大量滋阴补肾的药物配伍少量的桂枝、附子而成，以“阴中求阳”微微生火以治疗肾阳虚证。为分析肾气丸此种“阴中求阳”配伍治疗肾阳虚证的药效机理，本研究以氢化可的松制作大鼠肾阳虚模型，观察肾气丸及其各拆方组水煎液灌胃大鼠后，比较各组大鼠下丘脑神经递质多巴胺（DA）、五羟色胺（5-HT）的含量变化。现报道如下。 　　1 材料与仪器 　　1.1 动物健康SPF级雄性SD大鼠88只，体质量200g左右，广东省实验动物中心提供［质量合格证编号:0026197,许可证号：SCXK（粤）2003-0002 粤监证字2007A003］。正常