腺苷在肥胖大鼠中作用.docVIP

腺苷在肥胖大鼠中作用 　【摘要】 目的 探讨腺苷(CPA)在肥胖大鼠中的作用。方法 大鼠分为普通饲料喂养组（A组），高能饲料喂养肥胖模型组（B组），CPA＋高能饲料喂养模型组（C组）。检测大鼠脂肪重（g）/体重（g）、心脏重（g）/体重（g）、肾脏重（g）/体重（g）、中心静脉压、体动脉压，计算Lees指数。结果 (1)肥胖模型组大鼠体重、体脂/体重、Lees指数、中心静脉压（CVP）、体动脉压（BP）均高于普通饲料（P＜0.01、P＜0.05），而心、肝、肾与体重比低于普通饲料喂养组（P＜0.05）。(2)CPA加高能饲料喂养组体重、Lees指数、体脂/体重、CVP、BP低于肥胖组（P＜0.05），而与普通饲料组之间差异无显著性（P＞0.05）。(3)肥胖模型组大鼠心、肝、肾重与体重比小于普通饲料组、CPA加肥胖大鼠组（P＜0.05），而CPA加肥胖组与普通饲料组之间差异无显著性（P＞0.05）。结论 腺苷可能能抑制大鼠肥胖发生。　　【关键词】 腺苷；肥胖症；Lees指数 　　【Abstract】 Objective To observe the effects of adenosine(CPA)on obesity rats.Methods The rats were divided into three groups,one group were raised by normal food (group A),another were raised by high-energy food (group B)and high-energy food combined with CPA raised group(group C).The weight whole body were measured and the rate of heart/weight,fat/weight,liver/weight and kidney/weight,center vein pressure(CVP),blood pressure(BP) and Lee?s index were calculated respectively.Results (1)The body weight,the rate of fat/weight,Lee?s index,CVP,BP in high-energy food group were higher than those in normal groups(Plt;0.01，Plt;0.05),but the rate of heart/weight,liver/weight,kidney/weight were lower than those in normal groups(P＜0.05).(2)Body weight,Lees index,fat/weight,CVP,BP in high-energy food combined with CPA raised group were lower than those obesity rats group(P＜0.05),but it has no significant difference compared with normal raised group(P＞0.05).(3)The heart/weight,liver/weight and kidney/weight in obesity rats group were lower than those in normal raised group,high energy food combined with CPA raised group(P＜0.05),but those were no significant difference compared with normal raised group(P＞0.05).Conclusion Adenosine was likely to restrain the occurence of obesity rats. 　　【Key words】 adenosine；obesity；Lees index 　　腺苷（adenosine）是体内的一种重要的生物活性物质。腺苷根据受体的生物特性可分为A1、A2a、A2b、A3等3个亚型［1］。人们在白色脂肪细胞上发现A1腺苷受体。在白色脂肪细胞的培养液中分别加入A1腺苷受体特异性的激动剂CCPA和A1腺苷受体特异性的阻断药DPCPX后发现，加入CCPA的培养液中的脂肪细胞的生长受到了明显的抑制，加入DPCPX的培养液中的脂肪细胞生长良好［2］。本实验采用腺苷受体特异性的激动剂（N6-cyclo