色谱指纹图谱分析技术用于鉴别中药玄参产地探究.docVIP

色谱指纹图谱分析技术用于鉴别中药玄参产地探究 　 作者：马登峰 周浓 白志川 房立晶 孙婵娟 【摘要】 目的采用高效液相色谱（HPLC）色谱指纹图谱分析技术鉴别中药玄参产地。方法采用HPLC法测定了重庆市武隆县玄参等14个不同产地玄参样品。色谱条件C18柱，甲醇-1%冰醋酸为流动相进行梯度洗脱，检测波长290 nm，体积流量1.0 ml/min，柱温30℃。结果对重庆市武隆县10批次玄参样品的多个活性成分进行综合评价，筛选出重庆市道地药材玄参为建模对象，合成出玄参的标准HPLC色谱指纹图谱模型；并用此模型和特征成分的相对峰面积比值准确地区分浙江省磐安县等不同产地玄参。结论研究将有助于玄参药材的标准化种植及质量控制。 【关键词】 玄参 肉桂酸 高效液相色谱法 指纹图谱 质量控制 Abstract：ObjectiveTo identify the Habital of Radix Scrophulariae with Chromatographic Fingerprinting.MethodsActive constituents were extracted from ten batches of sample of Radix Scrophulariae (Scrophularia ningpoensis Hemsl.), which collected from Chongqing Wulong county, and evaluated by HPLC. Chromatographic conditions was Kromasil TM C18 column with methanol-1% glacial acetic acid as mobile phase, the detection wavelength was 290 nm, the flow rate was 1.0 ml·min-1, and column temperature was 30℃.ResultsMany active constituents that extracted from ten batches of sample of Radix Scrophulariae (Scrophularia ningpoensis Hemsl.), which collected from Chongqing Wulong county, were evaluated by HPLC. The models of standard HPLC Fingerprint of Radix Scrophulariae were established; Taking the cinnamic acid as the reference peak, the relative time and the relative peak area ration of various characteristics peak were calculated, and the sample from Zhejiang province could be distinguished by this model and the relative peak area ratio of the characteristic components.ConclusionThis study is good for the standard planting and quality control of Radix scrophulariae. Key words：Radix Scrophulariae（Scrophularia ningpoensis Hemsl.）; Cinnamic acid； HPLC； Fingerprint； Quality control 玄参科植物玄参Scrophularia ningpoensis Hemsl. 的干燥根具有凉血滋阴、泻火解毒的功效，临床上用于治疗热病伤阴，舌绛烦渴，温毒发斑，津伤便秘，骨蒸劳嗽，目赤，咽痛，瘰疬，白喉，痈肿疮毒［1］。玄参含哈巴苷、哈巴苷元、桃叶珊瑚苷、6-对甲基梓醇、浙玄参苷甲、乙等环烯醚萜类化合物及生物碱、植物甾醇、油酸、硬脂酸、葡萄糖、天冬酰胺、微量挥发油等［2］。《中国药典》2005年版Ⅰ部对玄参的质量控制采用HPLC法测定其中哈巴俄苷的含量［1］。众所周知，单一指标性成分的含量测定往往难以反映中药整体质量，而指纹图谱具有系统性、特征性和重现性特点，能较全面反映中药品质，现已成为中药质量评价和控制研究的热点之一［3～5］。本文以具有轻泻、抗细菌及真菌等作用的肉桂酸 ［6］作为指标成分，采用HPLC法构建不