蓬莪术药材指纹图谱探究.docVIP

蓬莪术药材指纹图谱探究 　作者：周欣， 陈华国，赵超，龚小见，李明 【摘要】 　　目的建立蓬莪术药材挥发性成分的气相色谱－质谱联用分析（GC-MS）指纹图谱，为蓬莪术药材的质量控制提供参考。方法采用水蒸气蒸馏法提取蓬莪术挥发油，用GC-MS联用技术对其进行指纹图谱测定，并且利用“中药色谱指纹图谱相似度评价软件”对不同样品之间的相似度进行计算。结果初步建立了蓬莪术挥发油的GC-MS指纹图谱，标定了20个共有峰，各批次药材均有上述特征，但特征峰的相对含量存在一定差异。结论该方法有良好的重现性和精密度，再加上GC-MS具有多成分同时定性的优势，所建立的蓬莪术挥发油的GC-MS指纹图谱为其质量控制提供了科学依据。 【关键词】 蓬莪术 挥发油 气相色谱-质谱联用 指纹图谱 　　Abstract：ObjectiveTo establish a method of fingerprint analysis on volatile oil of Curcuma phaeocaulis Valeton by GC-MS. MethodsThe essential oil of Curcuma phaeocaulis Valeton growing in Shuangliu city of Sichuan province was extracted by steam distillation and the chromatographic fingerprint was characterized by GC-MS. The GC-MS analysis was carried out in a Hewlett Packard model 6890 gas chromatograph coupled with a Hewlett Packard model 5973 mass detector. A Hewlett Packard HP-5 (5% phenyl methyl siloxane) fused silica capillary column (30 m×0.25 mm I. D., 0.25μm film thickness) was used for the separation. ResultsWe established Mutual fingerprint of the essential oil of Curcuma phaeocaulis Valeton by using the similarity calculation software of Chinese herbal fingerprint designed by Research Center of Modernization of Chinese Herbal Medicines of Central South University RSD of precision and reproducibility and stability of this GC-MS method was in the range of 5%. Compared with Mutual fingerprint of the essential oil of Curcuma phaeocaulis Valeton, the coefficients of 10 samples of the essential oil of Curcuma phaeocaulis Valeton were all above 90%, without abnormal sample.ConclusionThe method is reliable, accurate and can be used for quality control of essential oil of Curcuma phaeocaulis Valeton. 　　Key words：Curcuma phaeocaulis Valeton ; Essential oils; GC-MS; Fingerprint 　　莪术是姜科植物蓬莪术Curcuma phaeocaulis Valeton、广西莪术Curcuma kwangsinensis S.G.Lee et C.F. Liang和温莪术Curcuma wenyujin Y.H.Chen et C.Ling的干燥根茎。中医理论认为莪术辛、苦、温，归肝、脾经，具行气破血、消积止痛的功效［1］。莪术挥发油中的β-榄香烯对肿瘤细胞生长有直接抑制作用［2］。莪术油能抑制肿瘤细胞异常增殖［3］。 　　莪术油的质量直接关系到其制剂的质量，由于中药材的药效不是来自任何单一的