原创力文档a new mutant genetic resource for tomato crop improvement by tilling technology一个新的突变体遗传资源为番茄作物改良耕作技术.pdfVIP
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a new mutant genetic resource for tomato crop improvement by tilling technology一个新的突变体遗传资源为番茄作物改良耕作技术
Minoia et al. BMC Research Notes 2010, 3:69
/1756-0500/3/69
RESEARCH ARTICLE Open Access
A new mutant genetic resource for tomato crop
improvement by TILLING technology
1,3 1 1 2 1 1
Silvia Minoia , Angelo Petrozza , Olimpia D’Onofrio , Florence Piron , Giuseppina Mosca , Giovanni Sozio ,
1 2 1*
Francesco Cellini , Abdelhafid Bendahmane , Filomena Carriero
Abstract
Background: In the last decade, the availability of gene sequences of many plant species, including tomato, has
encouraged the development of strategies that do not rely on genetic transformation techniques (GMOs) for
imparting desired traits in crops. One of these new emerging technology is TILLING (Targeting Induced Local
Lesions In Genomes), a reverse genetics tool, which is proving to be very valuable in creating new traits in
different crop species.
Results: To apply TILLING to tomato, a new mutant collection was generated in the genetic background of the
processing tomato cultivar Red Setter by treating seeds with two different ethylemethane sulfonate doses (0.7%
and 1%). An associated phenotype database, LycoTILL, was developed and a TILLING platform was also established.
The interactive and evolving database is available online to the community for phenotypic alteration inquiries. To
validate the Red Setter TILLING platform, induced point mutations were searched in 7 tomato genes with the
mismatch-specific ENDO1 nuclease. In total 9.5 kb of tomato genome were screened and 66 nucleotide
substitutions were identified. The overall mutation density was estimated and it resulted to be 1/322 kb and
1/574 kb for the 1% EMS and 0.7% EMS treatment respectively.
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