a simplified method for analysis of polyunsaturated fatty acids一个简化的多不饱和脂肪酸的分析方法.pdfVIP

BMC Biochemistry BioMed Central Methodology article Open Access A simplified method for analysis of polyunsaturated fatty acids Jing X Kang* and Jingdong Wang Address: Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA Email: Jing X Kang* - jxkang@; Jingdong Wang - jwang18@ * Corresponding author Published: 24 March 2005 Received: 12 November 2004 Accepted: 24 March 2005 BMC Biochemistry 2005, 6:5 doi:10.1186/1471-2091-6-5 This article is available from: /1471-2091/6/5 © 2005 Kang and Wang; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Analysis of fatty acid composition of biological materials is a common task in lipid research. Conventionally, preparation of samples for fatty acid analysis by gas chromatography involves two separate procedures: lipid extraction and methylation. This conventional method is complicated, tedious and time consuming. Development of a rapid and simple method for lipid analysis is warranted. Results: We simplified the conventional method by combining the extraction and methylation into a single step (omitting the procedure of prior extraction). Various biological samples including cultured cells, animal tiss