a survey of mycoplasma agalactiae in dairy sheep farms in spain羊支原体的调查agalactiae在乳制品农场在西班牙.pdfVIP

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a survey of mycoplasma agalactiae in dairy sheep farms in spain羊支原体的调查agalactiae在乳制品农场在西班牙.pdf

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a survey of mycoplasma agalactiae in dairy sheep farms in spain羊支原体的调查agalactiae在乳制品农场在西班牙

A survey of Mycoplasma agalactiae in dairy sheep farms in Spain Ariza-Miguel et al. Ariza-Miguel et al. BMC Veterinary Research 2012, 8:171 /1746-6148/8/171 (24 September 2012) Ariza-Miguel et al. BMC Veterinary Research 2012, 8:171 /1746-6148/8/171 RESEARCH ARTICLE Open Access A survey of Mycoplasma agalactiae in dairy sheep farms in Spain * * Jaime Ariza-Miguel, David Rodríguez-Lázaro and Marta Hernández Abstract Background: Contagious Agalactia (CA) is one of the major animal health problems in small ruminants because of its economic significance. Currently, four Mycoplasma spp. have been associated with this syndrome: M. agalactiae, M. mycoides subsp. capri, M. capricolum subsp. capricolum and M. putrefaciens. Their presence has been evaluated in several studies conducted in CA-endemic countries. However, previous Spanish studies have been focused on caprine CA, and there is a knowledge gap regarding which Mycoplasma species are present in sheep flocks from Spain, which has the second highest number of sheep amongst the 27 European Union member states. Consequently, we investigated the presence and geographic distribution of the four CA-causing mycoplasmas in Spanish dairy sheep farms. This is the first time such an investigation has been performed. Results: Three hundred thirty nine out of 922 sheep flocks were positive for M. agalactiae by real time PCR (36.8%) and 85 by microbiological identification (9.2%). Interestingly, all 597 milk samples assessed for the presence of M. mycoides subsp. capri, M. capricolum subsp. capricolum and M. putrefaciens tested negative. To evaluate the