absence of pathogenic mitochondrial dna mutations in mouse brain tumors没有致病性线粒体dna突变老鼠脑部肿瘤.pdfVIP

BMC Cancer BioMed Central Research article Open Access Absence of pathogenic mitochondrial DNA mutations in mouse brain tumors Michael A Kiebish and Thomas N Seyfried* Address: Department of Biology, Boston College, Chestnut Hill, MA 02467 USA Email: Michael A Kiebish - Kiebish@; Thomas N Seyfried* - Thomas.Seyfried@ * Corresponding author Published: 16 August 2005 Received: 19 May 2005 Accepted: 16 August 2005 BMC Cancer 2005, 5:102 doi:10.1186/1471-2407-5-102 This article is available from: /1471-2407/5/102 © 2005 Kiebish and Seyfried; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Somatic mutations in the mitochondrial genome occur in numerous tumor types including brain tumors. These mutations are generally found in the hypervariable regions I and II of the displacement loop and unlikely alter mitochondrial function. Two hypervariable regions of mononucleotide repeats occur in the mouse mitochondrial genome, i.e., the origin of replication of the light strand (OL) and the Arg tRNA. Methods: In this study we examined the entire mitochondrial genome in a series of chemically induced brain tumors in the C57BL/6J strain and spontaneous brain tumors in the VM mouse strain. The tumor mtDNA