adaptation of hansenula polymorpha to methanol a transcriptome analysis改编自hansenula polymorpha甲醇转录组分析.pdfVIP

van Zutphen et al. BMC Genomics 2010, 11:1 /1471-2164/11/1 RESEARCH ARTICLE Open Access Adaptation of Hansenula polymorpha to methanol: a transcriptome analysis 1 1 1 2 2 Tim van Zutphen , Richard JS Baerends , Kim A Susanna , Anne de Jong , Oscar P Kuipers , Marten Veenhuis1,3, Ida J van der Klei1,3* Abstract Background: Methylotrophic yeast species (e.g. Hansenula polymorpha, Pichia pastoris) can grow on methanol as sole source of carbon and energy. These organisms are important cell factories for the production of recombinant proteins, but are also used in fundamental research as model organisms to study peroxisome biology. During exponential growth on glucose, cells of H. polymorpha typically contain a single, small peroxisome that is redundant for growth while on methanol multiple, enlarged peroxisomes are present. These organelles are crucial to support growth on methanol, as they contain key enzymes of methanol metabolism. In this study, changes in the transcriptional profiles during adaptation of H. polymorpha cells from glucose- to methanol-containing media were investigated using DNA-microarray analyses. Results: Two hours after the shift of cells from glucose to methanol nearly 20% (1184 genes) of the approximately 6000 annotated H. polymorpha genes were significantly upregulated with at least a two-fold differential expression. Highest upregulation ( 300-fold) was observed for the genes encoding the transcription factor Mpp1 and formate dehydrogenase, an enzyme of the methanol dissimilation pathway. Upregulated genes also included genes encoding other enzymes of methanol metabolism as well as of peroxisomal b-oxidation. A moderate increase in transcriptional levels (up to 4-fold) was observed for se