原创力文档adenoviral infectivity of exfoliated viable cells in urine implications for the detection of bladder canceradenoviral传染性剥落了可行的尿液细胞对膀胱癌的检测.pdfVIP
- 1、本文档共8页，可阅读全部内容。
- 2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
- 3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击 这里二次下载。
- 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
- 5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
- 6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
- 7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 8、VIP文档为合作方或网友上传，每下载1次， 网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
adenoviral infectivity of exfoliated viable cells in urine implications for the detection of bladder canceradenoviral传染性剥落了可行的尿液细胞对膀胱癌的检测
Murali et al. BMC Cancer 2011, 11:168
/1471-2407/11/168
RESEARCH ARTICLE Open Access
Adenoviral infectivity of exfoliated viable cells
in urine: Implications for the detection of
bladder cancer
† † *
Anuradha Murali , Laura Kasman and Christina Voelkel-Johnson
Abstract
Background: Bladder cancer, the 5th most common malignancy in the USA, is often detected as a result of
incidental findings or by presenting hematuria. Once diagnosed the disease is one of the costliest cancers to treat
due to frequent, invasive and often lifelong follow-up procedures. Because cells are shed into urine, there has been
an emerging effort to develop non-invasive tests for the detection of bladder cancer. Expression of survivin, a
member of the inhibitor of apoptosis protein family, has been associated with bladder cancer. Therefore, the goal
of this study was to determine the feasibility of transducing viable exfoliated cells obtained from urine with an
adenoviral vector in which a reporter gene is under the control of the survivin promoter.
Methods: Exfoliated cells from urine were obtained from 36 human subjects ( 40 years old). An adenovirus in
which GFP expression is under control of the survivin promoter (Ad.Surv.GFP) was generated. An adenovirus in
which GFP is expressed from the CMV promoter served as a control. GFP expression was analyzed by fluorescent
microscopy and quantified by flow cytometry.
Results: Short-term cultures from exfoliated cells in urine could be established in 16 of 31 samples. These cultures
were successfully transduced with Ad.CMV.GFP. Analysis of GFP expression following transduction with Ad.Surv.GFP,
indicated that the survivin promoter was preferentially active in UM-UC-3 bladder cancer cells compared to non-
您可能关注的文档
- acceptance and adverse effects of h1n1 vaccinations among a cohort of national guard health care workers during the 2009 hajj season接受和h1n1疫苗的副作用在一群国民警卫队的卫生保健工作者在2009年朝圣的季节.pdf
- acceptance of the who surgical safety checklist among surgical personnel in hospitals in guatemala city接受外科手术中世卫组织手术安全核对表的人员在医院在危地马拉城.pdf
- access and use of human tissues from the developing world ethical challenges and a way forward using a tissue trust访问和使用的人体组织来自发展中国家的伦理挑战和前进的使用组织的信任.pdf
- acceptance of oral chemotherapy in breast cancer patients - a survey study接受口服化疗在乳腺癌患者的一项调查研究.pdf
- acceptance and tolerability of an adjuvanted nh1n1 vaccine in hiv-infected patients in the cologne-bonn cohort接受和耐受性的含佐剂nh1n1疫苗在科隆-波恩的感染艾滋病毒的患者群.pdf
- access and care issues in urban urgent care clinic patients访问和问题在城市急诊治疗诊所的病人.pdf
- access to electronic health knowledge in five countries in africa a descriptive study访问非洲五个国家电子健康知识一个描述性的研究.pdf
- access to healthcare and alternative health-seeking strategies among undocumented migrants in denmark医疗条件和替代寻求卫生保健策略之间的非法移民在丹麦.pdf
- access to interpreting services in england secondary analysis of national data口译服务在英国中等国家数据的分析.pdf
- acceptability, effectiveness, and cost-effectiveness of internet-based exposure treatment for irritable bowel syndrome in a clinical sample a randomized controlled trial可接受性、有效性和成本效益网络暴露治疗肠易激综合征的临床随机对照试验样品.pdf
- adequate symptom relief justifies hepatic resection for benign disease足够的证明肝切除良性疾病的症状的缓解作用.pdf
- adenocarcinoma of the caecum metastatic to the bladder an unusual cause of haematuria盲肠的腺癌转移性血尿的膀胱一个不寻常的原因.pdf
- adequate sleep among adolescents is positively associated with health status and health-related behaviors充足的睡眠青少年与健康状况和健康相关行为呈正相关.pdf
- adenoviral expression of a transforming growth factor-β1 antisense mrna is effective in preventing liver fibrosis in bile-duct ligated ratsadenoviral转变增长factor-β1反义mrna的表达是有效预防胆管结扎大鼠肝纤维化.pdf
- adherence to cancer screening guidelines across canadian provinces an observational study坚持癌症筛查指南在加拿大省份一项观察性研究.pdf
- adherence to cardioprotective medications and mortality among patients with diabetes and ischemic heart disease坚持心血管药物,糖尿病和缺血性心脏病患者的死亡率.pdf
- adherence to isoniazid preventive therapy in indonesian children a quantitative and qualitative investigation坚持异烟肼预防性治疗在印尼儿童定量和定性调查.pdf
- adherence to anti-tuberculosis treatment among pulmonary tuberculosis patients a qualitative and quantitative study坚持抗结核治疗肺结核患者定性和定量研究.pdf
- adenovirus type 5 e1a-induced apoptosis in cox-2-overexpressing breast cancer cells5型腺病毒e1a-induced cox-2-overexpressing乳腺癌细胞凋亡.pdf
- adherence to colorectal cancer screening guidelines in canada坚持在加拿大结直肠癌筛查指标.pdf
文档评论（0）