an in vitro-identified high-affinity nucleosome-positioning signal is capable of transiently positioning a nucleosome in vivo的高亲和性nucleosome-positioning vitro-identified信号是瞬变体内定位一个核小体的能力.pdfVIP

Gracey et al. Epigenetics Chromatin 2010, 3:13 /content/3/1/13 RESEARCH Open Access An in vitro-identified high-affinity nucleosome-positioning signal is capable of transiently positioning a nucleosome in vivo 1 2 1 3 1,3 1,2* 1,3* Lia E Gracey , Zhi-Ying Chen , Jay M Maniar , Anton Valouev , Arend Sidow , Mark A Kay , Andrew Z Fire Abstract Background: The physiological function of eukaryotic DNA occurs in the context of nucleosomal arrays that can expose or obscure defined segments of the genome. Certain DNA sequences are capable of strongly positioning a nucleosome in vitro, suggesting the possibility that favorable intrinsic signals might reproducibly structure chromatin segments. As high-throughput sequencing analyses of nucleosome coverage in vitro and in vivo have become possible, a vigorous debate has arisen over the degree to which intrinsic DNA:nucleosome affinities orchestrate the in vivo positions of nucleosomes, thereby controlling physical accessibility of specific sequences in DNA. Results: We describe here the in vivo consequences of placing a synthetic high-affinity nucleosome-positioning signal, the 601 sequence, into a DNA plasmid vector in mice. Strikingly, the 601 sequence was sufficient to position nucleosomes during an early phase after introduction of the DNA into the mice (when the plasmid vector transgene was active). This positioning capability was transient, with a loss of strong positioning at a later time point when the transgenes had become silent. Conclusions: These results demonstrate an ability of DNA sequences selected solely for nucleosome affinity to organize chromatin in vivo, and the ability of other mechanisms to overcome these