an inter-platform repeatability study investigating real-time amplification of plasmid dnainter-platform重复性研究调查实时放大的质粒dna.pdfVIP

BMC Biotechnology BioMed Central Research article Open Access An inter-platform repeatability study investigating real-time amplification of plasmid DNA 1 1 1 2 Carol E Donald* , Fizza Qureshi , Malcolm J Burns , Marcia J Holden , 2 1 Joseph R Blasic Jr and Alison J Woolford Address: 1Bio-Molecular Innovation Team, LGC Ltd, Queens Road, Teddington, Middlesex TW11 0LY, UK and 2Bioprocess Measurement Group, National Institute of Standards and Technology (NIST), 100 Bureau Drive, Mail Stop 8312, Gaithersburg, MD 20899-8312, USA Email: Carol E Donald* - carol.donald@lgc.co.uk; Fizza Qureshi - carol.donald@lgc.co.uk; Malcolm J Burns - malcolm.burns@lgc.co.uk; Marcia J Holden - marcia.holden@; Joseph R Blasic - joseph.blasic@; Alison J Woolford - alison.woolford@lgc.co.uk * Corresponding author Published: 25 May 2005 Received: 06 October 2004 Accepted: 25 May 2005 BMC Biotechnology 2005, 5:15 doi:10.1186/1472-6750-5-15 This article is available from: /1472-6750/5/15 © 2005 Donald et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: The wide variety of real-time amplification platforms currently availab