原创力文档analysis of escherichia coli nicotinate mononucleotide adenylyltransferase mutants in vivo and in vitro分析烟酸大肠杆菌单核苷酸adenylyltransferase突变体在体内和体外.pdfVIP
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BMC Biochemistry BioMed Central
Research article Open Access
Analysis of Escherichia coli nicotinate mononucleotide
adenylyltransferase mutants in vivo and in vitro
2 3 1
Martin Stancek , Robert Schnell and Monica Rydén-Aulin*
Address: 1Department of Genetics, Microbiology and Toxicology, Stockholm University, S-106 91 Stockholm, Sweden, 2In vitro Sweden AB, Box
21160, S-100 31 Stockholm, Sweden and 3Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S-171 77 Stockholm,
Sweden
Email: Martin Stancek - martin.stancek@invitro.se; Robert Schnell - robert.schnell@mbb.ki.se; Monica Rydén-Aulin* - monica@gmt.su.se
* Corresponding author
Published: 09 September 2005 Received: 07 June 2005
Accepted: 09 September 2005
BMC Biochemistry 2005, 6:16 doi:10.1186/1471-2091-6-16
This article is available from: /1471-2091/6/16
© 2005 Stancek et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0),
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Background: Adenylation of nicotinate mononucleotide to nicotinate adenine dinucleotide is the
penultimate step in NAD+ synthesis. In Escherichia coli, the enzyme nicotinate mononucleotide
adenylyltransferase is encoded by the nadD gene. We have earlier made an initial characte
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