原创力文档analysis of transcript and protein overlap in a human osteosarcoma cell line转录和蛋白质分析重叠在一个人类骨肉瘤细胞系.pdfVIP
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analysis of transcript and protein overlap in a human osteosarcoma cell line转录和蛋白质分析重叠在一个人类骨肉瘤细胞系
Klevebring et al. BMC Genomics 2010, 11:684
/1471-2164/11/684
RESEARCH ARTICLE Open Access
Analysis of transcript and protein overlap in
a human osteosarcoma cell line
1,3 2 2 1 2 1*
Daniel Klevebring , Linn Fagerberg , Emma Lundberg , Olof Emanuelsson , Mathias Uhlén , Joakim Lundeberg
Abstract
Background: An interesting field of research in genomics and proteomics is to compare the overlap between the
transcriptome and the proteome. Recently, the tools to analyse gene and protein expression on a whole-genome
scale have been improved, including the availability of the new generation sequencing instruments and high-
throughput antibody-based methods to analyze the presence and localization of proteins. In this study, we used
massive transcriptome sequencing (RNA-seq) to investigate the transcriptome of a human osteosarcoma cell line
and compared the expression levels with in situ protein data obtained in-situ from antibody-based
immunohistochemistry (IHC) and immunofluorescence microscopy (IF).
Results: A large-scale analysis based on 2749 genes was performed, corresponding to approximately 13% of the
protein coding genes in the human genome. We found the presence of both RNA and proteins to a large fraction
of the analyzed genes with 60% of the analyzed human genes detected by all three methods. Only 34 genes
(1.2%) were not detected on the transcriptional or protein level with any method. Our data suggest that the
majority of the human genes are expressed at detectable transcript or protein levels in this cell line. Since the
reliability of antibodies depends on possible cross-reactivity, we compared the RNA and protein data using
antibodies with different reliability scores based on
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