原创力文档analytical variables influencing the performance of a mirna based laboratory assay for prediction of relapse in stage i non-small cell lung cancer (nsclc)分析变量影响microrna的实验室试验为基础的性能预测复发的i期非小细胞肺癌(nsclc).pdfVIP
- 1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
- 2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击 这里二次下载。
- 3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
- 4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
- 5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
- 6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 7、VIP文档为合作方或网友上传，每下载1次， 网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
Dahlgaard et al. BMC Research Notes 2011, 4:424
/1756-0500/4/424
RESEARCH ARTICLE Open Access
Analytical variables influencing the performance
of a miRNA based laboratory assay for prediction
of relapse in stage I non-small cell lung cancer
(NSCLC)
1,5* 1 1 3,4 6 1 2
Jesper Dahlgaard , Wiktor Mazin , Thomas Jensen , Mette Pøhl , Wiam Bshara , Anker Hansen , Eric Kanisto ,
5 3 5 3,4 2
Stephen Jacques Hamilton-Dutoit , Olfred Hansen , Henrik Hager , Henrik J Ditzel , Sai Yendamuri and
Steen Knudsen1
Abstract
Background: Laboratory assays are needed for early stage non-small lung cancer (NSCLC) that can link molecular
and clinical heterogeneity to predict relapse after surgical resection. We technically validated two miRNA assays for
prediction of relapse in NSCLC. Total RNA from seventy-five formalin-fixed and paraffin-embedded (FFPE)
specimens was extracted, labeled and hybridized to Affymetrix miRNA arrays using different RNA input amounts,
ATP-mix dilutions, array lots and RNA extraction- and labeling methods in a total of 166 hybridizations. Two
combinations of RNA extraction- and labeling methods (assays I and II) were applied to a cohort of 68 early stage
NSCLC patients.
Results: RNA input amount and RNA extraction- and labeling methods affected signal intensity and the number of
detected probes and probe sets, and caused large variation, whereas different ATP-mix dilutions and array lots did
not. Leave-one-out accuracies for prediction of relapse were 63% and 73% for the two assays. Prognosticator calls
(no recurrence” or “recurrence”) were co
您可能关注的文档
- an ovary transcriptome for all maturational stages of the striped bass (morone saxatilis), a highly advanced perciform fish一个卵巢转录组的所有成熟阶段条纹鲈鱼(马龙saxatilis),一个高度先进的perciform鱼.pdf
- an overiew of non medical prescribing across one strategic health authority a questionnaire survey非医疗处方的overiew卫生战略部门一个问卷调查.pdf
- an outbreak of post-acupuncture cutaneous infection due to mycobacterium abscessus的爆发由于脓肿分枝杆菌post-acupuncture皮肤感染.pdf
- an overlooked connection serotonergic mediation of estrogen-related physiology and pathology忽略连接estrogen-related生理学和病理学的血清素激活的中介.pdf
- an original serpina3 gene cluster elucidation of genomic organization and gene expression in the bos taurus 21q24 region一个原始serpina3基因簇说明基因组组织和基因表达的牛21抓起.pdf
- an overview of ethical frameworks in public health can they be supportive in the evaluation of programs to prevent overweight公共卫生伦理框架的概述可以支持的评估程序,以防止超重.pdf
- an overview of the cellml api and its implementationcellml api的概述及其实现.pdf
- an svm-based system for predicting protein subnuclear localizations基于系统预测蛋白质的亚核的本地化.pdf
- an r package variabel for genome-wide searching of potentially interacting loci by testing genotypic variance heterogeneityr包variabel全基因组搜索潜在的相互作用位点的检测基因型方差异质性.pdf
- an rna isolation system for plant tissues rich in secondary metabolitesrna隔离系统对植物组织丰富的次生代谢产物.pdf
- analyzing stochastic transcription to elucidate the nucleoid's organization分析随机转录阐明类核的组织.pdf
- analyzing fixed points of intracellular regulation networks with interrelated feedback topology分析细胞内调控网络的不动点和拓扑相关的反馈.pdf
- analyzing generic and branded substitution patterns in the netherlands using prescription data分析通用和品牌使用处方数据替换模式在荷兰.pdf
- analysis on multi-domain cooperation for predicting protein-protein interactions多域合作分析预测蛋白质相互作用.pdf
- analysis of thisbe and pyramus functional domains reveals evidence for cleavage of drosophila fgfs提斯柏和皮拉摩斯功能域的分析,将揭示果蝇fgf乳沟的证据.pdf
- analyzing 2d gel images using a two-component empirical bayes model使用双组分分析二维凝胶图像经验贝叶斯模型.pdf
- analysis on conservation of disulphide bonds and their structural features in homologous protein domain families分析保护二硫化物债券及其在同源蛋白质域结构特点的家庭.pdf
- analyzing the gene expression profile of pediatric acute myeloid leukemia with real-time pcr arrays分析儿童急性髓系白血病的基因表达谱与实时pcr数组.pdf
- analyzing mirna co-expression networks to explore tf-mirna regulation分析microrna co-expression网络探索tf-mirna监管.pdf
- analysis of x-ray structures of matrix metalloproteinases via chaotic map clustering分析x射线结构基质金属蛋白酶通过混沌映射聚类.pdf
文档评论（0）