angiotensin ii receptor expression and relation to helicobacter pylori-infection in the stomach of the mongolian gerbil血管紧张素ⅱ受体表达和关系在长爪沙鼠的胃幽门螺杆菌感染.pdfVIP

Hallersund et al. BMC Gastroenterology 2010, 10:3 /1471-230X/10/3 RESEARCH ARTICLE Open Access Angiotensin II receptor expression and relation to Helicobacter pylori-infection in the stomach of the Mongolian gerbil * Peter Hallersund , Herbert F Helander, Anna Casselbrant, Anders Edebo, Lars Fändriks, Anders Elfvin Abstract Background: The role of the renin-angiotensin system in gastric physiology and disease has as yet been sparsely explored. The first aim of the study was to investigate the baseline presence and location of angiotensin II receptors (AT1R and AT2R) in the stomach of the Mongolian gerbil. A second aim was to elucidate whether the presence of H. pylori infection is associated with changes in the expression of these receptors. Methods: H. pylori-negative and H. pylori-infected (strain SS1 or TN2GF4) male Mongolian gerbils were investigated. The stomachs were examined at six or 12 months after inoculation by the use of immunohistochemistry, western blot and microscopic morphometry. Results: AT1R and AT2R were located in a variety of cells in the gerbil gastric wall, including a subpopulation of endocrine cells in the antral mucosa and inflammatory cells infiltrating H. pylori-infected stomachs. Gerbils infected with the SS1 strain showed a significantly increased antral AT1R protein expression and an increased number of infiltrating polymorphonuclear leucocytes (PMNs) at 12 months. The AT1R protein expression correlated with the number of PMNs and the antral expression of myeloperoxidase. Conclusions: Angiotensin II receptors are present in a variety of cells in the gastric wall of the Mongolian gerbil. The results indicate an influence dependent on the H. pylori strain on the gastric AT1R expression and a relationship between gastric AT1R expression and mucosal PMNs infiltratio