anti-inflammatory and antioxidant effects of tualang honey in alkali injury on the eyes of rabbits experimental animal studytualang蜂蜜的抗炎和抗氧化效果碱损伤眼睛的兔子实验动物研究.pdfVIP

Bashkaran et al. BMC Complementary and Alternative Medicine 2011, 11:90 /1472-6882/11/90 RESEARCH ARTICLE Open Access Anti-inflammatory and antioxidant effects of Tualang honey in alkali injury on the eyes of rabbits: Experimental animal study 1 1* 2 3 4 Karuppannan Bashkaran , Embong Zunaina , Shaharuddin Bakiah , Siti Amrah Sulaiman , KNS Sirajudeen and Venkatesh Naik5 Abstract Background: Alkali injury is one of the most devastating injuries to the eye. It results in permanent unilateral or bilateral visual impairment. Chemical eye injury is accompanied by an increase in the oxidative stress. Anti- inflammatory and antioxidant agents play a major role in the treatment of chemical eye injuries. The purpose of this study is to evaluate the anti-inflammatory (clinical and histopathological) and antioxidant effects of Tualang honey versus conventional treatment in alkali injury on the eyes of rabbits. Methods: A preliminary study was carried out prior to the actual study to establish the alkali chemical injury on rabbit’s cornea and we found that alkali chemical injury with 2 N NaOH showed severe clinical inflammatory features. In actual study, alkali injury with 2 N NaOH was induced in the right eye of 10 New Zealand White rabbits’ cornea. The rabbits were divided into two groups, Group A was given conventional treatment and Group B was treated with both topical and oral Tualang honey. Clinical inflammatory features of the right eye were recorded at 12 hours, 24 hours, 72 hours, 5th day and 7th day post induction of alkali burn on the cornea. The histopathological inflammatory features of the right corneas of all rabbits were also evaluated on day-7. The level of t