antimicrobial and antioxidant properties of methanol extract, fractions and compounds from the stem bark of entada abyssinica stend ex a. satabie抗菌和抗氧化性能的甲醇提取物,分数和化合物的干树皮entada abyssinica stend a . satabie交货.pdfVIP

Teke et al. BMC Complementary and Alternative Medicine 2011, 11:57 /1472-6882/11/57 RESEARCH ARTICLE Open Access Antimicrobial and antioxidant properties of methanol extract, fractions and compounds from the stem bark of Entada abyssinica Stend ex A. Satabie 1 1 2 1* 3 3 Gerald N Teke , Paul K Lunga , Hippolyte K Wabo , Jules-Roger Kuiate , Gerard Vilarem , Geraldine Giacinti , Haruhisa Kikuchi4 and Yoshiteru Oshima4 Abstract Background: The aim of this study was to evaluate the antimicrobial and antioxidant activities of the methanol extract, fractions and isolated compounds from Entada abyssinica stem bark, plant used traditionally against gastrointestinal infections. Methods: The methanol extract of E. abyssinica stem bark was pre-dissolved in a mixture of methanol and water, and then partitioned between n-hexane, ethyl acetate and n-butanol. The ethyl acetate portion was fractionated by column chromatography and the structures of isolated compounds elucidated by analysis of spectroscopic data and comparison with literature data. Antimicrobial activity was assayed by broth microdilution techniques on bacteria and yeasts. The antioxidant activity was determined by DPPH radical scavenging method. Results: Four known compounds [(5S,6R,8aR)-5-(carboxymethyl)-3,4,4a,5,6,7,8,8a-octahydro-5,6,8a- trimethylnaphthalenecarboxylic acid (1), methyl 3,4,5-trihydroxybenzoate (2), benzene-1,2,3-triol (3) and 2,3- dihydroxypropyltriacontanoate (4)] were isolated. Compared to the methanol extract, fractionation increased the antibacterial activities of the n-hexane and ethyl acetate fractions, while the antifungal activities increased in ethyl acetate, n-butanol and aqueous residue fractions