原创力文档array comparative genomic hybridization analysis of trichoderma reesei strains with enhanced cellulase production properties阵列比较基因组杂交分析,木霉属reesei与增强的纤维素酶生产菌株的特性.pdfVIP
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array comparative genomic hybridization analysis of trichoderma reesei strains with enhanced cellulase production properties阵列比较基因组杂交分析,木霉属reesei与增强的纤维素酶生产菌株的特性
Vitikainen et al. BMC Genomics 2010, 11:441
/1471-2164/11/441
R E S E A R C H A R T I C L E Open Access
Research article
Array comparative genomic hybridization analysis
of Trichoderma reesei strains with enhanced
cellulase production properties
Marika Vitikainen*, Mikko Arvas, Tiina Pakula, Merja Oja, Merja Penttilä and Markku Saloheimo
Abstract
Background: Trichoderma reesei is the main industrial producer of cellulases and hemicellulases that are used to
depolymerize biomass in a variety of biotechnical applications. Many of the production strains currently in use have
been generated by classical mutagenesis. In this study we characterized genomic alterations in high-producing
mutants of T. reesei by high-resolution array comparative genomic hybridization (aCGH). Our aim was to obtain
genome-wide information which could be utilized for better understanding of the mechanisms underlying efficient
cellulase production, and would enable targeted genetic engineering for improved production of proteins in general.
Results: We carried out an aCGH analysis of four high-producing strains (QM9123, QM9414, NG14 and Rut-C30) using
the natural isolate QM6a as a reference. In QM9123 and QM9414 we detected a total of 44 previously undocumented
mutation sites including deletions, chromosomal translocation breakpoints and single nucleotide mutations. In NG14
and Rut-C30 we detected 126 mutations of which 17 were new mutations not documented previously. Among these
new mutations are the first chromosomal translocation breakpoints identified in NG14 and Rut-C30. We studied the
effects of two deletions identified in Rut-C30 (a deletion of 85 kb in the scaffold 15 and a deletion in a gene encoding a
transcription factor) on cellulase production by constructing knock-out strains in the QM6a background. Neither the 85
kb deletion nor the de
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