array comparative hybridisation reveals a high degree of similarity between uk and european clinical isolates of hypervirulent clostridium difficile阵列杂交显示高度的相似性比较英国和欧洲hypervirulent艰难梭状芽胞杆菌的临床分离株.pdfVIP

Marsden et al. BMC Genomics 2010, 11:389 /1471-2164/11/389 R E S E A R C H A R T I C L E Open Access Research article Array comparative hybridisation reveals a high degree of similarity between UK and European clinical isolates of hypervirulent Clostridium difficile 1,4 1 1 2 3 1 Gemma L Marsden , Ian J Davis , Victoria J Wright , Mohammed Sebaihia , Ed J Kuijper and Nigel P Minton* Abstract Background: Clostridium difficile is a Gram-positive, anaerobic, spore-forming bacterium that is responsible for C. difficile associated disease in humans and is currently the most common cause of nosocomial diarrhoea in the western world. This current status has been linked to the emergence of a highly virulent PCR-ribotype 027 strain. The aim of this work was to identify regions of sequence divergence that may be used as genetic markers of hypervirulent PCR- ribotype 027 strains and markers of the sequenced strain, CD630 by array comparative hybridisation. Results: In this study, we examined 94 clinical strains of the most common PCR-ribotypes isolated in mainland Europe and the UK by array comparative genomic hybridisation. Our array was comprehensive with 40,097 oligonucleotides covering the C. difficile 630 genome and revealed a core genome for all the strains of 32%. The array also covered genes unique to two PCR-ribotype 027 strains, relative to C. difficile 630 which were represented by 681 probes. All of these genes were also found in the commonly occuring PCR-ribotypes 001 and 106, and the emerging hypervirulent PCR- ribotype 078 strains, indicating that these are markers for all highly virulent strains. Conclusions: We have fulfilled the aims of this study by identifying markers for CD630 a