assessment of the genetic diversity of the tunisian citrus rootstock germplasm评估突尼斯柑橘砧木种质资源的遗传多样性.pdfVIP

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assessment of the genetic diversity of the tunisian citrus rootstock germplasm评估突尼斯柑橘砧木种质资源的遗传多样性.pdf

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assessment of the genetic diversity of the tunisian citrus rootstock germplasm评估突尼斯柑橘砧木种质资源的遗传多样性

Snoussi et al. BMC Genetics 2012, 13:16 /1471-2156/13/16 RESEARCH ARTICLE Open Access Assessment of the genetic diversity of the Tunisian citrus rootstock germplasm 1* 2 3 1 2 2 Hager Snoussi , Marie-France Duval , Andres Garcia-Lor , Zina Belfalah , Yann Froelicher , Ange-Marie Risterucci , 2 2 3 4 2,3* Xavier Perrier , Jean-Pierre Jacquemoud-Collet , Luis Navarro , Moncef Harrabi and Patrick Ollitrault Abstract Background: Citrus represents a substantial income for farmers in the Mediterranean Basin. However, the Mediterranean citrus industry faces increasing biotic and abiotic constraints. Therefore the breeding and selection of new rootstocks are now of the utmost importance. In Tunisia, in addition to sour orange, the most widespread traditional rootstock of the Mediterranean area, other citrus rootstocks and well adapted to local environmental conditions, are traditionally used and should be important genetic resources for breeding. To characterize the diversity of Tunisian citrus rootstocks, two hundred and one local accessions belonging to four facultative apomictic species (C. aurantium, sour orange; C. sinensis, orange; C. limon, lemon; and C. aurantifolia, lime) were collected and genotyped using 20 nuclear SSR markers and four indel mitochondrial markers. Multi-locus genotypes (MLGs) were compared to references from French and Spanish collections. Results: The differentiation of the four varietal groups was well-marked. The groups displayed a relatively high allelic diversity, primarily due to very high heterozygosity. Sixteen dist