原创力文档electron tomography of negatively stained complex viruses application in their diagnosis电子断层扫描负染色复杂的病毒在其诊断中的应用.pdfVIP
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electron tomography of negatively stained complex viruses application in their diagnosis电子断层扫描负染色复杂的病毒在其诊断中的应用
Diagnostic Pathology BioMed Central
Research Open Access
Electron tomography of negatively stained complex viruses:
application in their diagnosis
Jan Mast* and Lien Demeestere
Address: EM-unit, CODA-CERVA, Groeselenberg 99, Brussels, Belgium
Email: Jan Mast* - jamas@var.fgov.be; Lien Demeestere - lidem@var.fgov.be
* Corresponding author
Published: 10 February 2009 Received: 13 January 2009
Accepted: 10 February 2009
Diagnostic Pathology 2009, 4:5 doi:10.1186/1746-1596-4-5
This article is available from: /content/4/1/5
© 2009 Mast and Demeestere; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0),
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Background: Electron tomographic analysis can be combined with the simple and rapid negative
staining technique used in electron microscopy based virus diagnosis.
Methods: Standard negative staining of representative examples of parapoxviruses and
paramyxoviruses was combined with electron tomographic analysis.
Results: Digital sectioning of reconstructions of these viruses at a selected height demonstrated
the viral ultrastructure in detail, including the characteristic diagnostic features like the surface
threads on C-particles of a parapoxvirus and individual glycoproteins and the in
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