autoantibodies to aberrantly glycosylated muc1 in early stage breast cancer are associated with a better prognosis自身抗体异常糖化muc1在早期乳腺癌与更好的预后有关.pdfVIP

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autoantibodies to aberrantly glycosylated muc1 in early stage breast cancer are associated with a better prognosis自身抗体异常糖化muc1在早期乳腺癌与更好的预后有关.pdf

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autoantibodies to aberrantly glycosylated muc1 in early stage breast cancer are associated with a better prognosis自身抗体异常糖化muc1在早期乳腺癌与更好的预后有关

Blixt et al. Breast Cancer Research 2011, 13:R25 /content/13/2/R25 RESEARCH ARTICLE Open Access Autoantibodies to aberrantly glycosylated MUC1 in early stage breast cancer are associated with a better prognosis 1* 2 3,4 2,5 2 6 Ola Blixt , Deanna Bueti , Brian Burford , Diane Allen , Sylvain Julien , Michael Hollingsworth , 3 5 2* 2* Alex Gammerman , Ian Fentiman , Joyce Taylor-Papadimitriou and Joy M Burchell Abstract Introduction: Detection of serum biomarkers for early diagnosis of breast cancer remains an important goal. Changes in the structure of O-linked glycans occur in all breast cancers resulting in the expression of glycoproteins that are antigenically distinct. Indeed, the serum assay widely used for monitoring disease progression in breast cancer (CA15.3), detects a glycoprotein (MUC1), but elevated levels of the antigen cannot be detected in early stage patients. However, since the immune system acts to amplify the antigenic signal, antibodies can be detected in sera long before the antigen. We have exploited the change in O-glycosylation to measure autoantibody responses to cancer-associated glycoforms of MUC1 in sera from early stage breast cancer patients. Methods: We used a microarray platform of 60mer MUC1 glycopeptides, to confirm the presence of autoantibodies to cancer associated glycoforms of MUC1 in a proportion of early breast cancer patients (54/198). Five positive sera were selected for detailed definition of the reactive epitopes using on chip glycosylation technology and a panel of glycopeptides based on a single MUC1 tandem repeat carrying