histopathological and parasitological study of the gastrointestinal tract of dogs naturally infected with leishmania infantum组织病理学和寄生虫学的研究胃肠道的狗自然感染婴儿利什曼虫.pdfVIP

Pinto et al. Acta Veterinaria Scandinavica 2011, 53:67 /content/53/1/67 RESEARCH Open Access Histopathological and parasitological study of the gastrointestinal tract of dogs naturally infected with Leishmania infantum 1† 1† 2 1 3 Aldair JW Pinto , Maria M Figueiredo , Fabiana L Silva , Trycia Martins , Marilene SM Michalick , Washington L Tafuri4 and Wagner L Tafuri1* Abstract Background: The aim of this study was to provide a systematic pathological and parasitological overview of the gastrointestinal tract (GIT), including the stomach, duodenum, jejunum, ileum, caecum and colon, of dogs naturally infected with Leishmania. Methods: Twenty mongrel dogs naturally infected with Leishmania (Leishmania) infantum and obtained from the Control Zoonosis Center of the Municipality of Ribeirão das Neves, Belo Horizonte Metropolitan area, Minas Gerais (MG) state, Brazil, were analyzed. The dogs were divided into two groups: Group 1 comprised nine clinically normal dogs and group 2 comprised 11 clinically affected dogs. After necropsy, one sample was collected from each GIT segment, namely the stomach, duodenum, jejunum, ileum, caecum and colon. Furthermore, paraffin-embedded samples were used for histological and parasitological (immunohistochemistry) evaluation and a morphometrical study were carried out to determine the parasite load (immunolabeled amastigote forms of Leishmania). The Friedman and the Mann Whitney tests were used for statistical analysis. The Friedman test was used to analyze each segment of the GIT within each group of dogs and the Mann Whitney test was used to compare the GIT segments between clinically unaffected and affected dogs. Results: The infected dogs had an increa