hiv-1 resistance conferred by sirna cosuppression of cxcr4 and ccr5 coreceptors by a bispecific lentiviral vector抗hiv - 1的sirna cosuppression授予由双特异性趋化因子受体cxcr4与ccr5 coreceptors慢病毒载体.pdfVIP

AIDS Research and Therapy BioMed Central Research Open Access HIV-1 resistance conferred by siRNA cosuppression of CXCR4 and CCR5 coreceptors by a bispecific lentiviral vector Joseph Anderson and Ramesh Akkina* Address: Dept Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA Email: Joseph Anderson - lacrosse@; Ramesh Akkina* - akkina@ * Corresponding author Published: 13 January 2005 Received: 26 October 2004 Accepted: 13 January 2005 AIDS Research and Therapy 2005, 2:1 doi:10.1186/1742-6405-2-1 This article is available from: /content/2/1/1 © 2005 Anderson and Akkina; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. HIV/AIDS gene therapyHIV-1 co-receptorsCCR5 siRNACXCR4 siRNABispecific Lentiviral vector Abstract Background: RNA interference (RNAi) mediated by small interfering RNAs (siRNAs) has proved to be a highly effective gene silencing mechanism with great potential for HIV/AIDS gene therapy. Previous work with siRNAs against cellular coreceptors CXCR4 and CCR5 had shown that down regulation of these surface molecules could prevent HIV-1 entry and confer viral resistance. Since monospecific siRNAs targeting individual coreceptors are inadequate in protecting against both T cell tropic (X4) and monocyte trop