intracellular atp assay of live cells using ptd-conjugated luciferase活细胞的细胞内atp测定使用ptd-conjugated荧光素酶.pdfVIP

Sensors 2012, 12, 15628-15637; doi:10.3390/s121115628 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article Intracellular ATP Assay of Live Cells Using PTD-Conjugated Luciferase Mi-Sook Lee 1,2, Wan-Soon Park 1, Young Han Kim 1, Won Gyeong Ahn 1, Seung-Hae Kwon 1 and Song Her 1,* 1 Division of Bio-Imaging, Chuncheon Center, Korea Basic Science Institute, Chuncheon 200-701, Korea; E-Mails: mslee0621@ (M.-S.L.); wans8@kbsi.re.kr (W.-S.P.); yhkim10@kbsi.re.kr (Y.H.K.); wgahn09@kbsi.re.kr (W.G.A.); kwonsh@kbsi.re.kr (S.-H.K.) 2 Acupuncture Meridian Science Research Center, College of Oriental Medicine, Kyung Hee University, Seoul 130-701, Korea * Author to whom correspondence should be addressed; E-Mail: swher@kbsi.re.kr; Tel.: +82-33-250-7394; Fax: +82-33-255-7273. Received: 27 September 2012; in revised form: 8 November 2012 / Accepted: 8 November 2012 / Published: 12 November 2012 Abstract: Luciferase is a sensitive, reliable biological sensor used for measuring ATP. However, its widespread application in drug discovery and toxicology studies has been limited due to unavoidable cell extraction processes, which cause inaccurate measurements of intracellular ATP and obstruct the application of homogenous high-throughput screening. Recently, we developed a protein transduction domain-conjugated luciferase (PTD-Luc) for measuring cellular uptake efficacy. In this study, we evaluated the applicability of