molecular mechanism of arenavirus assembly and budding分子机制的沙粒病毒装配和萌芽.pdfVIP

Viruses 2012, 4, 2049-2079; doi:10.3390/v4 102049 OPEN ACCESS viruses ISSN 1999-4915 /journal/viruses Review Molecular Mechanism of Arenavirus Assembly and Budding Shuzo Urata and Jiro Yasuda * Department of Emerging Infectious Diseases, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan; E-Mail: shuzourata@nagasaki-u.ac.jp * Author to whom correspondence should be addressed; E-Mail: j -yasuda@nagasaki-u.ac.jp; Tel.: +81-95-819-7848; Fax: +81-95-819-7851. Received: 3 August 2012; in revised form: 25 September 2012 / Accepted: 28 September 2012 / Published: 10 October 2012 Abstract: Arenaviruses have a bisegmented negative-strand RNA genome, which encodes four viral proteins: GP and NP by the S segment and L and Z by the L segment. These four viral proteins possess multiple functions in infection, replication and release of progeny viruses from infected cells. The small RING finger protein, Z protein is a matrix protein that plays a central role in viral assembly and budding. Although all arenaviruses encode Z protein, amino acid sequence alignment showed a huge variety among the species, especially at the C-terminus where the L-domain is located. Recent publications have demonstrated the interactions between viral protein and viral protein, and viral protein and host cellular protein, which facilitate transportation and assembly of viral components to sites of virus egress. This