molecular properties of water-unextractable proteoglycans from hypsizygus marmoreus and their in vitro immunomodulatory activities分子的性质water-unextractable蛋白聚糖从hypsizygus体外及其体外免疫调节活动.pdfVIP

Molecules 2012, 17, 207-226; doi:10.3390/molecules OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Molecular Properties of Water-Unextractable Proteoglycans from Hypsizygus marmoreus and Their in Vitro Immunomodulatory Activities Hong Hui Bao 1,†, Mehdi Tarbasa 1, Hee Mun Chae 2,* and Sang Guan You 1,* 1 Department of Marine Food Science and Technology, Gangneung-Wonju National University, 120 Gangneung Daehangno, Gangneung, Gangwon, 210-702, Korea E-Mails: honghui_bao@163.com (H.H.B.); mtabarsa@ (M.T.) 2 Climate Change Research Institute of Korea, Chuncheon-si, Gangwon-do, 200-939, Korea † Present address: College of Food Science, Heilongjiang Bayi Agricultural University, Heilongjiang, Daqing 163319, China. * Authors to whom correspondence should be addressed; E-Mails: cheemun6606@ (H.M.C.); umyousg@gwnu.ac.kr (S.G.Y.); Tel.: +82-33-254-2103 (H.M.C.); +82-33-640-2853 (S.G.Y.); Fax: +82-33-254-2014 (H.M.C.); +82-33-640-2340 (S.G.Y.). Received: 27 October 2011; in revised form: 7 December 2011 / Accepted: 22 December 2011 / Published: 27 December 2011 Abstract: Four proteoglycans were sequentially extracted from Hypsizygus marmoreus using 0.1 M NaOH (alkali-soluble proteoglycans [F1] and alkali-insoluble proteoglycans [F3]) and 0.1 M HCl (acid-soluble proteoglycans [F2] and acid-insoluble proteoglycans [F4]), and their structures and immunomodulatory activities were investigated. The proteoglycans were found to contain carbohydrates (19.8–82.4%) with various amounts of proteins