interleukin 2-regulated in vitro antibody production following a single spinal manipulative treatment in normal subjects白介素2-regulated体外抗体生产单后脊髓操纵在正常受试者治疗.pdfVIP

Teodorczyk-Injeyan et al. Chiropractic Osteopathy 2010, 18:26 /content/18/1/26 RESEARCH Open Access Interleukin 2-regulated in vitro antibody production following a single spinal manipulative treatment in normal subjects 1 2 3 4* Julita A Teodorczyk-Injeyan , Marion McGregor , Richard Ruegg , H Stephen Injeyan Abstract Background: Our recent investigations have demonstrated that cell cultures from subjects, who received a single spinal manipulative treatment in the upper thoracic spine, show increased capacity for the production of the key immunoregulatory cytokine, interleukin-2. However, it has not been determined if such changes influence the response of the immune effector cells. Thus, the purpose of the present study was to determine whether, in the same subjects, spinal manipulation-related augmentation of the in vitro interleukin-2 synthesis is associated with the modulation of interleukin 2-dependent and/or interleukin-2-induced humoral immune response (antibody synthesis). Methods: A total of seventy-four age and sex-matched healthy asymptomatic subjects were studied. The subjects were assigned randomly to: venipuncture control (n = 22), spinal manipulative treatment without cavitation (n = 25) or spinal manipulative treatment associated with cavitation (n = 27) groups. Heparinized blood samples were obtained from the subjects before (baseline) and then at 20 minutes and 2 hours post-treatment. Immunoglobulin (antibody) synthesis was induced in cultures of peripheral blood mononuclear cells by stimulation with conventional pokeweed mitogen or by application of human recombinant interleukin-2. Determinations of the levels of immunoglobulin G and immunoglobulin M production in culture supernatants w