原创力文档interleukin-6 regulates anti-arthritic effect of methotrexate via reduction of slc19a1 expression in a mouse arthritis model白细胞介素- 6调节抗风湿作用通过减少甲氨蝶呤slc19a1表达小鼠关节炎模型.pdfVIP
- 1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
- 2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击 这里二次下载。
- 3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
- 4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
- 5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
- 6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 7、VIP文档为合作方或网友上传，每下载1次， 网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
interleukin-6 regulates anti-arthritic effect of methotrexate via reduction of slc19a1 expression in a mouse arthritis model白细胞介素- 6调节抗风湿作用通过减少甲氨蝶呤slc19a1表达小鼠关节炎模型
Hashizume et al. Arthritis Research Therapy 2012, 14:R96
/content/14/2/R96
RESEARCH ARTICLE Open Access
Interleukin-6 regulates anti-arthritic effect of
methotrexate via reduction of SLC19A1
expression in a mouse arthritis model
1* 1 1 1 2 2
Misato Hashizume , Hiroto Yoshida , Keisuke Tanaka , Miho Suzuki , Isao Matsumoto , Takayuki Sumida and
Masahiko Mihara1
Abstract
Introduction: Methotrexate (MTX) enters cells via the reduced folate carrier SLC19A1, suggesting that SLC19A1 is
associated with the efficacy of MTX. We here examined the relationship between the efficacy of MTX and the
expression of SLC19A1 in glucose 6-phosphate isomerase (GPI)-induced arthritis. We found that interleukin-6 (IL-6)
regulated the expression of SLC19A1, so we studied the effect of a combination of MTX and anti-mouse IL-6
receptor antibody (MR16-1).
Methods: GPI-induced arthritis was induced by intradermal immunization with recombinant GPI. MTX was given
from the first day of immunization. Mice were injected once with MR16-1 10 days after immunization. The levels of
SLC19A1 mRNA in whole hind limbs and immune cells were measured. Synovial cells from arthritic mice were
cultured with cytokines, and cell proliferation and gene expressions were measured.
Results: MTX inhibited the development of GPI-induced arthritis; however, the efficacy of MTX gradually
diminished. SLC19A1 expression in immunized mice with arthritis was lower than in intact mice; moreover,
SLC19A1 expression in arthritic mice was further decreased when they were treated with MTX. IL-6 was highly
expressed in whole hind limbs of arthritic mice. In an in vitro study using synovial cells from arthritic mice, IL-6 +
soluble IL-
您可能关注的文档
- hiv-1 ribonuclease h structure, catalytic mechanism and inhibitorshiv - 1 h核糖核酸酶的结构、催化机理和抑制剂.pdf
- hiv as a chronic disease considerations for service planning in resource-poor settings艾滋病作为慢性疾病注意事项服务计划在资源贫乏的环境中.pdf
- history of the world allergy organization a global scientific meeting on allergy comes to asia--the xx world allergy congress of bangkok, thailand, 2007历史世界过敏组织全球过敏科学会议涉及到亚洲的xx世界过敏大会曼谷,泰国,2007年.pdf
- hiv-1 virological synapse live imaging of transmissionhiv - 1病毒学突触传播实时成像.pdf
- hiv-2 diagnosis and quantification in high-risk patientshiv - 2高危患者的诊断和量化.pdf
- hiv-1 protease structural perspectives on drug resistancehiv - 1蛋白酶结构角度对耐药性.pdf
- hivaids among youth in urban informal (slum) settlements in kenya what are the correlates of and motivations for hiv testing条件青年在城市非正式定居点(贫民窟)在肯尼亚的关联和动机进行艾滋病毒检测.pdf
- hiv-1 mutational pathways under multidrug therapyhiv - 1突变通路在耐多药治疗.pdf
- hivaids mitigation strategies and the state in sub-saharan africa – the missing link条件缓和策略和国家在撒哈拉以南非洲地区u2014u2014缺失的环节.pdf
- hiv-1 virological synapse is not simply a copycat of the immunological synapsehiv - 1病毒学突触不仅仅是免疫突触的模仿.pdf
- interleukin-6 discovery of a pleiotropic cytokine多效性的细胞因子白细胞介素- 6的发现.pdf
- interleukin-6 and chronic inflammation白细胞介素- 6和慢性炎症.pdf
- interleukin-7 deficiency in rheumatoid arthritis consequences for therapy-induced lymphopenia白介素- 7缺乏对治疗导致风湿性关节炎影响淋巴细胞减少.pdf
- interleukin-13 signaling and its role in asthmainterleukin-13信号及其在哮喘中的作用.pdf
- interleukin-12 peripheral blood levels in asthmatic childreninterleukin-12哮喘儿童外周血水平.pdf
- interleukin-17 (il-17) expression is reduced during acute myocardial infarction role on chemokine receptor expression in monocytes and their in vitro chemotaxis towards chemokinesinterleukin-17(il-17)中表达降低急性心肌梗死的作用在单核细胞趋化因子受体的表达及其在体外对趋化因子趋化性.pdf
- interleukin-15 and interferon-γ participate in the cross-talk between natural killer and monocytic cells required for tumour necrosis factor productioninterleukin-15 interferon-γ参与之间的相声所需自然杀伤细胞和单核细胞的细胞肿瘤坏死因子生产.pdf
- interleukin-7-aggravated joint inflammation and tissue destruction in collagen-induced arthritis is associated with t-cell and b-cell activationinterleukin-7-aggravated关节炎症和组织破坏胶原诱导关节炎与t细胞和b细胞活化相关.pdf
- interleukin-10 gene down-expression in circulating mononuclear cells during infusion of drotrecogin-α activated a pilot study在循环单核细胞白细胞介素- 10u201d基因抑制注入drotrecogin-α激活一个试点研究.pdf
- interleukin-18 as an in vivo mediator of monocyte recruitment in rodent models of rheumatoid arthritisinterleukin-18单核细胞的体内中介招聘在类风湿性关节炎的动物模型.pdf
文档评论（0）