原创力文档molecular weight and monosaccharide composition of astragalus polysaccharides分子量和黄芪多糖的单糖组成.pdfVIP
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molecular weight and monosaccharide composition of astragalus polysaccharides分子量和黄芪多糖的单糖组成
Molecules 2008, 13, 2408-2415; DOI: 10.3390/molecule
OPEN ACCESS
molecules
ISSN 1420-3049
/molecules
Article
Molecular Weight and Monosaccharide Composition of
Astragalus Polysaccharides
Du-Juan Xu 1,*, Quan Xia 1, Jia-Jia Wang 2 and Pei-Pei Wang 2
1 Department of Pharmacy, the First Affiliated Hospital of Anhui Medical University, Hefei, 230022.
P. R. China; E-mail: xiaquan@ (Q. X.)
2 School of Pharmacy, Anhui Medical University, Hefei, 230032. P. R. China;
E-mails: wjj1982211@ (J-J. W.),wangpeipei2001@ (P-P. W.)
* Author to whom correspondence should be addressed. E-mail: xudujuan6365@;
Fax: (+86)-551-292 2442; Tel: (+86)-551-292 2442.
Received: 22 August 2008; in revised form: 24 September 2008 / Accepted: 26 September 2008 /
Published: 1 October 2008
Abstract: Two polysaccharides (APS-I and APS-II) were isolated from the water extract
of Radix Astragali and purified through ethanol precipitation, deproteination and by ion-
exchange and gel-filtration chromatography. Their molecular weight was determined using
high performance liquid chromatography and gel permeation chromatography (HPLC-
GPC) and their monosaccharide composition was analyzed by TLC and HPLC methods,
using a refractive index detector (RID) and an NH2 column. It was shown that APS-I
consisted of arabinose and glucose and APS-II consisted of rhamnose, arabinose and
glucose, in a molar ratio of 1:3.45 and 1:6.25:17.86, respectively. The molecular weights
(Mw) of APS-I and APS-II were 1
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