molecular species identification of central european ground beetles (coleoptera carabidae) using nuclear rdna expansion segments and dna barcodes分子物种鉴定中欧地面甲虫(鞘翅目步行虫科)使用核rdna扩张段和dna条形码.pdfVIP

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molecular species identification of central european ground beetles (coleoptera carabidae) using nuclear rdna expansion segments and dna barcodes分子物种鉴定中欧地面甲虫(鞘翅目步行虫科)使用核rdna扩张段和dna条形码.pdf

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molecular species identification of central european ground beetles (coleoptera carabidae) using nuclear rdna expansion segments and dna barcodes分子物种鉴定中欧地面甲虫(鞘翅目步行虫科)使用核rdna扩张段和dna条形码

Raupach et al. Frontiers in Zoology 2010, 7:26 /content/7/1/26 RESEARCH Open Access Molecular species identification of Central European ground beetles (Coleoptera: Carabidae) using nuclear rDNA expansion segments and DNA barcodes 1,2* 1 3 1 4 Michael J Raupach , Jonas J Astrin , Karsten Hannig , Marcell K Peters , Mark Y Stoeckle , Johann-Wolfgang Wägele1 Abstract Background: The identification of vast numbers of unknown organisms using DNA sequences becomes more and more important in ecological and biodiversity studies. In this context, a fragment of the mitochondrial cytochrome c oxidase I (COI) gene has been proposed as standard DNA barcoding marker for the identification of organisms. Limitations of the COI barcoding approach can arise from its single-locus identification system, the effect of introgression events, incomplete lineage sorting, numts, heteroplasmy and maternal inheritance of intracellular endosymbionts. Consequently, the analysis of a supplementary nuclear marker system could be advantageous. Results: We tested the effectiveness of the COI barcoding region and of three nuclear ribosomal expansion segments in discriminating ground beetles of Central Europe, a diverse and well-studied invertebrate taxon. As nuclear markers we determined the 18S rDNA: V4, 18S rDNA: V7 and 28S rDNA: D3 expansion segments for 344 specimens of 75 species. Seventy-three species (97%) of the analysed species could be accurately identified using COI, while the combined approach of all three nuclear markers provided resolution among 71 (95%) of the studied Carabidae. Conclusion: Our results confirm that the analysed nuclear ribosomal expansion segments in combination constitute a valuable and efficient suppleme