interferon-lambda1 induces peripheral blood mononuclear cell-derived chemokines secretion in patients with systemic lupus erythematosus its correlation with disease activityinterferon-lambda1诱发外周血单核细胞衍生趋化因子的分泌在系统性红斑狼疮患者与疾病活动的相关性.pdfVIP

Wu et al. Arthritis Research Therapy 2011, 13:R88 /content/13/3/R88 RESEARCH ARTICLE Open Access Interferon-lambda1 induces peripheral blood mononuclear cell-derived chemokines secretion in patients with systemic lupus erythematosus: its correlation with disease activity 1 1 2 1 1* Qian Wu , Qingrui Yang , Elaine Lourenco , Hongsheng Sun and Yuanchao Zhang Abstract Introduction: Systemic lupus erythematosus (SLE) is an autoimmune disease involving multiple organ systems. Previous studies have suggested that interferon-lambda 1 (IFN-l 1), a type III interferon, plays an immunomodulatory role. In this study we investigated its role in SLE, including its correlation with disease activity, organ disorder and production of chemokines. Methods: We determined levels of IFN-l 1 mRNA in peripheral blood mononuclear cells (PBMC) and serum protein levels in patients with SLE using real-time polymerase chain reaction (real-time PCR) and enzyme-linked immunoassay (ELISA). Further, we detected the concentration of IFN-inducible protein-10 (IP-10), monokine induced by IFN-g (MIG) and interleukin-8 (IL-8) secreted by PBMC under the stimulation of IFN-l 1 using ELISA. Results: IFN-l 1 mRNA and serum protein levels were higher in patients with SLE compared with healthy controls. Patients with active disease showed higher IFN-l 1 mRNA and serum protein levels compared with those with inactive disease as well. Serum IFN-l 1 levels were positively correlated with Systemic Lupus Erythematosus Disease Activity Index (SLEDAI), anti-dsDNA antibody, C-reactive protein (CRP) and negatively correlated with complement 3. Serum IFN-l 1 levels were higher in SLE patients with renal involvement and arthritis compared with patients wi