oestrogen is important for maintenance of cartilage and subchondral bone in a murine model of knee osteoarthritis雌激素对软骨和软骨下骨的维护很重要在小鼠模型的骨关节炎.pdfVIP

Sniekers et al. Arthritis Research Therapy 2010, 12:R182 /content/12/5/R182 RESEARCH ARTICLE Open Access Oestrogen is important for maintenance of cartilage and subchondral bone in a murine model of knee osteoarthritis 1,2 1 1,3 2* Yvonne H Sniekers , Harrie Weinans , Gerjo JVM van Osch , Johannes PTM van Leeuwen Abstract Introduction: Oestrogen depletion may influence onset and/or progression of osteoarthritis. We investigated in an ovariectomized mouse model the impact of oestrogen loss and oestrogen supplementation on articular cartilage and subchondral bone in tibia and patella, and assessed bone changes in osteoarthritis development. Methods: C3H/HeJ mice were divided into four groups: sham-operated, oestrogen depletion by ovariectomy (OVX), OVX with estradiol supplementation (OVX+E) and OVX with bisphosphonate (OVX+BP). Each mouse had one knee injected with low-dose iodoacetate (IA), and the contralateral knee was injected with saline. Cartilage was analysed histologically 12 weeks postsurgery; bone changes were monitored over time using in vivo micro- computed tomography. Results: In tibiae, OVX alone failed to induce cartilage damage, but OVX and IA combination significantly induced cartilage damage. In patellae, OVX alone induced significant cartilage damage, which was enhanced by IA. In both tibiae and patellae, OVX in combination with IA significantly decreased subchondral cortical thickness in an additive manner. OVX+E and OVX+BP inhibited tibial and patellar subchondral cortical thinning, inhibited patellar and tended to diminish tibial cartilage damage. In patellae, IA interacted with BP, leading to increased subchondral cortical and trabecular bone. Conclusions: This study de