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Polymers 2012, 4, 1535-1553; doi:10.3390/polym4031535 OPEN ACCESS polymers ISSN 2073-4360 /journal/polymers Article Physical Properties of Polypeptide Electrospun Nanofiber Cell Culture Scaffolds on a Wettable Substrate Donald T. Haynie 1,2,*, Dhan B. Khadka 1 and Michael C. Cross 1 1 Nanomedicine and Nanobiotechnology Laboratory, Department of Physics, University of South Florida, 4202 East Fowler Avenue, Tampa, FL 33620, USA; E-Mails: dkhadka@ (D.B.K.); mcross80@ (M.C.C) 2 Center for Integrated Functional Materials, Department of Physics, University of South Florida, 4202 East Fowler Avenue, Tampa, FL 33620, USA * Author to whom correspondence should be addressed; E-Mail: dhaynie@; Tel.: +1-813-974-7793; Fax: +1-813-974-5813. Received: 20 April 2012; in revised form: 9 August 2012 / Accepted: 23 August 2012 / Published: 31 August 2012 Abstract: Physical properties of poly(L-ornithine) (PLO), a polycation, poly(L-glutamic acid - 4 co-L-tyrosine) (PLEY), a polyanion, and electrospun fibers made of these polymers have been determined and compared. The polymers adopted random coil-like conformations in aqueous feedstocks at neutral pH and in dehydrated cast films and fibers on glass, and the fibers comprised numerous counterions, according to spectral analysis. Adsorption of model proteins and serum proteins onto hydrated and crosslinked fibers depended on the electrical charge of the