pigs taking wing with transposons and recombinases猪与转座子翼和可.pdfVIP

Review Pigs taking wing with transposons and recombinases Karl J Clark*†‡¤, Daniel F Carlson*†¤ and Scott C Fahrenkrug*†‡ *Department of Animal Science at the University of Minnesota, Fitch Ave, St. Paul, MN 55108, USA. †The Arnold and Mabel Beckman Center for Transposon Research at the University of Minnesota, Church St, Minneapolis, MN 55455, USA. ‡The Animal Biotechnology Center at the University of Minnesota, Fitch Ave, St. Paul, MN 55108, USA. ¤These authors contributed equally to this work. Correspondence: Scott Fahrenkrug. E-mail: fahre001@ Published: 31 October 2007 Genome Biology 2007, 8(Suppl 1):S13 (doi:10.1186/gb-2007-8-S1-S13) The electronic version of this article is the complete one and can be found online at /2007/8/S1/S13 © 2007 BioMed Central Ltd Abstract Swine production has been an important part of our lives since the late Mesolithic or early Neolithic periods, and ranks number one in world meat production. Pig production also contributes to high-value-added medical markets in the form of pharmaceuticals, heart valves, and surgical materials. Genetic engineering, including the addition of exogenous genetic material or manipulation of the endogenous genome, holds great promise for changing pig phenotypes for agricultural and medical applications. Although the first transgenic pigs were described in 1985, poor survival of manipulated embryos; inefficiencies in the integration, transmission, and expression of transgenes; and expensive husbandry costs have impeded the widespread application of pig genetic engineering. Sequencing of the pig genome and advances in reproductive technologies have rejuvenated efforts to apply t