plasma creatinine in dogs intra- and inter-laboratory variation in 10 european veterinary laboratories血浆肌酐狗内部在10个欧洲兽医实验室,实验室内部变化.pdfVIP

Ulleberg et al. Acta Veterinaria Scandinavica 2011, 53:25 /content/53/1/25 RESEARCH Open Access Plasma creatinine in dogs: intra- and inter- laboratory variation in 10 European veterinary laboratories 1 2 3 4 5* Tina Ulleberg , Joris Robben , Kathrine M Nordahl , Thomas Ulleberg and Reidun Heiene Abstract Background: There is substantial variation in reported reference intervals for canine plasma creatinine among veterinary laboratories, thereby influencing the clinical assessment of analytical results. The aims of the study was to determine the inter- and intra-laboratory variation in plasma creatinine among 10 veterinary laboratories, and to compare results from each laboratory with the upper limit of its reference interval. Methods: Samples were collected from 10 healthy dogs, 10 dogs with expected intermediate plasma creatinine concentrations, and 10 dogs with azotemia. Overlap was observed for the first two groups. The 30 samples were divided into 3 batches and shipped in random order by postal delivery for plasma creatinine determination. Statistical testing was performed in accordance with ISO standard methodology. Results: Inter- and intra-laboratory variation was clinically acceptable as plasma creatinine values for most samples were usually of the same magnitude. A few extreme outliers caused three laboratories to fail statistical testing for consistency. Laboratory sample means above or below the overall sample mean, did not unequivocally reflect high or low reference intervals in that laboratory. Conclusions: In spite of close analytical results, further standardization among laboratories is warranted. The discrepant reference intervals seem to largely reflect differe