preparation of silver nanoparticle and its application to the determination of ct-dna银纳米颗粒的制备及其应用ct-dna的决心.pdf

Sensors 2007, 7, 708-718 sensors ISSN 1424-8220 © 2007 by MDPI /sensors Full Research Paper Preparation of Silver Nanoparticle and Its Application to the Determination of ct-DNA Chunhua Liu 1, Xiupei Yang 1,3, Hongyan Yuan 2, Zaide Zhou 1 and Dan Xiao 1,2* 1 College of Chemistry, Sichuan University, Chengdu 610064, P. R. China 2 College of Chemical Engineering, Sichuan University, Chengdu 610064, P. R. China 3 College of Chemistry and Chemical Engineering, China West Normal University, Nanchong 637002, P. R. China * Author to whom correspondence should be addressed; Fax +86 28 Tel. +86 28 E-mail: xiaodan@ Received: 16 March 2007 / Accepted: 29 May 2007 / Published: 31 May 2007 Abstract: A new method to prepare silver nanoparticles was reported in this paper, which based on aqueous-gaseous phase reaction of silver nitrate solution and ammonia gas. The proposed method is effective, rapid, and convenient. Transmission electron microscope (TEM) and X-ray diffraction (XRD) were used to characterize the structure of the particles, respectively. It was found that the average diameter of the particles was about 10 nm and the shape was spherical. And the strong fluorescence signal of the silver nanoparticles solution can be quenched after the adding of the calf thymus DNA (ct-DNA) solution. The quenched fluorescence intensity was linear with the concentration of ct-DNA in the range of 4 -1 -1 0