preservation of bacillus firmus strain 37 and optimization of cyclodextrin biosynthesis by cells immobilized on loofa sponge保护杆菌firmus应变37和优化环糊精生物合成的细胞固定化loofa海绵.pdfVIP

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preservation of bacillus firmus strain 37 and optimization of cyclodextrin biosynthesis by cells immobilized on loofa sponge保护杆菌firmus应变37和优化环糊精生物合成的细胞固定化loofa海绵.pdf

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preservation of bacillus firmus strain 37 and optimization of cyclodextrin biosynthesis by cells immobilized on loofa sponge保护杆菌firmus应变37和优化环糊精生物合成的细胞固定化loofa海绵

Molecules 2012, 17, 9476-9488; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Preservation of Bacillus firmus Strain 37 and Optimization of Cyclodextrin Biosynthesis by Cells Immobilized on Loofa Sponge Rúbia Pazzetto 1, Sabrina Barbosa de Souza Ferreira 1, Elder James Silva Santos 1, Cristiane Moriwaki 1, Teresinha Aparecida Guedes 2 and Graciette Matioli 1,* 1 Department of Pharmacy, State University of Maringá (UEM), Av. Colombo, 5790, Maringá-PR 87020-900, Brazil 2 Department of Statistics, State University of Maringá (UEM), Av. Colombo, 5790, Maringá-PR 87020-900, Brazil * Author to whom correspondence should be addressed; E-Mail: gmatioli@uem.br; Tel.: +55-44-3011-3868; Fax: +55-44-3011-4119. Received: 28 April 2012; in revised form: 18 July 2012 / Accepted: 21 July 2012 / Published: 8 August 2012 Abstract: The preservation of Bacillus firmus strain 37 cells by lyophilization was evaluated and response surface methodology (RSM) was used to optimize the β-cyclodextrin (β-CD) production by cells immobilized on loofa sponge. Interactions were studied with the variables temperature, pH and dextrin concentration using a central composite design (CCD). Immobilization time influence on β-CD production was also investigated. B. firmus strain 37 cells remained viable after one year of storage, showing that the lyophilization is a suitable method for preservation of the microorganism. From the three-dimensional diagrams and