production optimization of cyanophycinase chpeal from pseudomonas alcaligenes dip1生产优化cyanophycinase chpeal从假单胞菌产碱杆菌属dip1.pdfVIP

Sallam et al. AMB Express 2011, 1:38 /content/1/1/38 ORIGINAL Open Access Production optimization of cyanophycinase ChpEal from Pseudomonas alcaligenes DIP1 * Ahmed Sallam, Dimitar Kalkandzhiev and Alexander Steinbüchel Abstract Pseudomonas alcaligenes DIP1 produces an extracellular cyanophycinase (CphE ). The corresponding gene (cphE ) al al was identified from subclones of a genomic DNA gene library by heterologously expressing the functionally active enzyme in Escherichia coli. The nucleotide sequence of the gene (1260 base pairs) was determined indicating a theoretical mass of 43.6 kDa (mature CphEal) plus a leader peptide of 2,6 kDa which corresponds well to the apparent molecular mass of 45 kDa as revealed by SDS. The enzyme exhibited a high sequence identity of 91% with the extracellular cyanophycinase from P. anguilliseptica strain BI and carried an N-terminal Sec secretion signal peptide. Analysis of the amino acid sequence of cphE revealed a putative catalytic triad consisting of the serine motif GXSXG plus a histidine and a glutamate residue, suggesting a catalytic mechanism similar to serine- type proteases. The cyanophycinase (CphEal) was heterologously produced in two different E. coli strains (Top10 and BL21(DE3)) from two plasmid vectors (pBBR1MCS-4 and pET-23a(+)). The signal peptide of CphEal was cleaved in E. coli, suggesting active export of the protein at least to the periplasm. Substantial enzyme activity was also present in the culture supernatants. The extracellular cyanophycinase activities in E. coli were higher than activities in the wild type P. alcaligenes