relaxation of adaptive evolution during the hiv-1 infection owing to reduction of cd4+ t cell counts放松的适应性进化在hiv - 1感染由于cd4 + t细胞数量的减少.pdfVIP

Relaxation of Adaptive Evolution during the HIV-1 Infection Owing to Reduction of CD4+ T Cell Counts ´ 1 2 3 4 5 Elcio Leal *, Jorge Casseb , Michael Hendry , Michael P. Busch , Ricardo Sobhie Diaz ´ ´ ˜ ˜ 1 Biotechnology Institute, Federal University of Para, Belem, Brazil, 2 Institute of Tropical Medicine of Sao Paulo, University of Sao Paulo, Sao Paulo, SP, Brazil, 3 Centers for Disease Control and Prevention (CDC), Laboratory Branch, Atlanta, United States of America, 4 Dept. of Laboratory Medicine, University of California San Francisco; Blood ˜ ˜ Systems Research Institute; Blood Systems, Inc. San Francisco, United States of America, 5 Federal University of Sao Paulo, Sao Paulo, Brazil Abstract Background: The first stages of HIV-1 infection are essential to establish the diversity of virus population within host. It has been suggested that adaptation to host cells and antibody evasion are the leading forces driving HIV evolution at the initial stages of AIDS infection. In order to gain more insights on adaptive HIV-1 evolution, the genetic diversity was evaluated during the infection time in individuals contaminated by the same viral source in an epidemic cluster. Multiple sequences of V3 loop region of the HIV-1 were serially sampled from four individuals: comprising a single blood donor, two blood recipients, and another sexually infected by one of the blood recipients. The diversity of the viral population within each host was analyzed independently in distinct time points during