原创力文档reduction of non-specific protein adsorption using poly(ethylene) glycol (peg) modified polyacrylate hydrogels in immunoassays for staphylococcal enterotoxin b detection减少非特异性蛋白吸附使用聚(乙烯)乙二醇(peg)的改性聚丙烯酸酯水凝胶在免疫测定葡萄球菌肠毒素b检测.pdfVIP
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reduction of non-specific protein adsorption using poly(ethylene) glycol (peg) modified polyacrylate hydrogels in immunoassays for staphylococcal enterotoxin b detection减少非特异性蛋白吸附使用聚(乙烯)乙二醇(peg)的改性聚丙烯酸酯水凝胶在免疫测定葡萄球菌肠毒素b检测
Sensors 2009, 9, 645-655; doi:10.3390/
OPEN ACCESS
sensors
ISSN 1424-8220
/journal/sensors
Article
Reduction of Non-Specific Protein Adsorption Using
Poly(ethylene) Glycol (PEG) Modified Polyacrylate Hydrogels
In Immunoassays for Staphylococcal Enterotoxin B Detection
Paul T. Charles*, Veronte R. Stubbs, Carissa M. Soto, Brett D. Martin, Brandy J. White and
Chris R. Taitt
Center for Bio/Molecular Science and Engineering (Code 6920), US Naval Research Laboratory, 4555
Overlook Ave. SW, Washington, DC 20375, U.S.A.
* Author to whom correspondence should be addressed; E-Mail: paul.charles@;
Tel.: +1-(202) 404-6064; Fax: +1-(202) 767-1980
Received: 31 October 2008; in revised form: 14 January 2009 / Accepted: 20 January 2009 /
Published: 23 January 2009
Abstract: Three PEG molecules (PEG-methacrylate, -diacrylate and -dimethacrylate) were
incorporated into galactose-based polyacrylate hydrogels and their relative abilities to
reduce non-specific protein adsorption in immunoassays were determined. Highly
crosslinked hydrogels containing amine-terminated functionalities were formed and used
to covalently attach antibodies specific for staphylococcal enterotoxin B (SEB). Patterned
arrays of immobilized antibodies in the PEG-modified hydrogels were created with a
PDMS template containing micro-channels for use in sandwich immunoassays to detect
SEB. Different concentrations of the toxin were applied to the hydrogel arrays, followed
with a Cy3-labeled t
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