原创力文档rescue of recent virulent and avirulent field strains of bluetongue virus by reverse genetics救援最近致命的、无毒的反向遗传学领域的蓝舌病病毒株.pdfVIP
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rescue of recent virulent and avirulent field strains of bluetongue virus by reverse genetics救援最近致命的、无毒的反向遗传学领域的蓝舌病病毒株
Rescue of Recent Virulent and Avirulent Field Strains of
Bluetongue Virus by Reverse Genetics
´ 1 1 2¤ 2¤
Rene G. P. van Gennip *, Sandra G. P. van de Water , Christiaan A. Potgieter , Isabel M. Wright , Daniel
Veldman1, Piet A. van Rijn1
1 Department of Virology, Central Veterinary Institute of Wageningen UR (CVI), Lelystad, The Netherlands, 2 Onderstepoort Veterinary Institute, Onderstepoort, South
Africa
Abstract
Since 1998, Bluetongue virus (BTV)-serotypes 1, 2, 4, 9, and 16 have invaded European countries around the Mediterranean
Basin. In 2006, a huge BT-outbreak started after incursion of BTV-serotype 8 (BTV8) in North-Western Europe. More recently,
BTV6 and BTV11 were reported in North-Western Europe in 2008. These latter strains are closely related to live-attenuated
vaccine, whereas BTV8 is virulent and can induce severe disease in ruminants, including cattle. In addition, Toggenburg
orbivirus (TOV) was detected in 2008 in Swiss goats, which was recognized as a new serotype of BTV (BTV25). The (re-
)emergency of known and unknown BTV-serotypes needs a rapid response to supply effective vaccines, and research to
study this phenomenon. Recently, orbivirus research achieved an important breakthrough by the establishment of reverse
genetics for BTV1. Here, reverse genetics for two recent BTV strains representing virulent BTV8 and avirulent BTV6 was
developed. For this purpose, extensive sequencing of full-genomes was performed, resulting in the consensus sequences of
BTV8/net07 and BTV6/net08. The recovery of ‘synthetic BTV’, respectively rgBTV8 and rgBTV6, completely from T7-derived
RNA transcripts was confirmed by silent mutations by which these ‘
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